Diagnostic value of meat juice in early detection of classical swine fever infection

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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@article{17e35e183b57411596463a72eef997bb,
title = "Diagnostic value of meat juice in early detection of classical swine fever infection",
keywords = "Swine, Reverse transcription polymerase chain reaction, Meat juice, Classical swine fever virus",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
author = "Louise Lohse and Åse Uttenthal and Rasmussen, {Thomas Bruun} and Jens Nielsen",
year = "2011",
doi = "10.1177/1040638711416855",
volume = "23",
number = "5",
pages = "1005--1008",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",

}

RIS

TY - JOUR

T1 - Diagnostic value of meat juice in early detection of classical swine fever infection

A1 - Lohse,Louise

A1 - Uttenthal,Åse

A1 - Rasmussen,Thomas Bruun

A1 - Nielsen,Jens

AU - Lohse,Louise

AU - Uttenthal,Åse

AU - Rasmussen,Thomas Bruun

AU - Nielsen,Jens

PB - American Association of Veterinary Laboratory Diagnosticians

PY - 2011

Y1 - 2011

N2 - To evaluate the diagnostic potential of meat juice for early detection of Classical swine fever virus (CSFV), meat juice and serum samples from pigs experimentally infected with different strains of CSFV were compared for virus load. From all samples, viral RNA was extracted by automated procedure before real-time reverse transcription polymerase chain reaction analysis was performed. Viral RNA was detected in meat juice, but at a lower level than in corresponding serum. Sensitivity was calculated to 91% and specificity to 97%. Disagreements between meat juice and serum results were found when samples originated from pigs infected with low virulence CSFV strains and/or when samples were collected within the first days after infection. In conclusion, while not the first choice for sample material for CSFV diagnosis, meat juice may constitute a useful alternative for herd-based studies or when blood and/or target organ material is not available. Strain virulence and time points for sample collection after infection are factors of importance for diagnostic success.

AB - To evaluate the diagnostic potential of meat juice for early detection of Classical swine fever virus (CSFV), meat juice and serum samples from pigs experimentally infected with different strains of CSFV were compared for virus load. From all samples, viral RNA was extracted by automated procedure before real-time reverse transcription polymerase chain reaction analysis was performed. Viral RNA was detected in meat juice, but at a lower level than in corresponding serum. Sensitivity was calculated to 91% and specificity to 97%. Disagreements between meat juice and serum results were found when samples originated from pigs infected with low virulence CSFV strains and/or when samples were collected within the first days after infection. In conclusion, while not the first choice for sample material for CSFV diagnosis, meat juice may constitute a useful alternative for herd-based studies or when blood and/or target organ material is not available. Strain virulence and time points for sample collection after infection are factors of importance for diagnostic success.

KW - Swine

KW - Reverse transcription polymerase chain reaction

KW - Meat juice

KW - Classical swine fever virus

U2 - 10.1177/1040638711416855

DO - 10.1177/1040638711416855

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 5

VL - 23

SP - 1005

EP - 1008

ER -