Standard

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{71e4a40269c244d3b0e56c1849d0a0a5,
title = "Diagnostic capacity for viral haemorrhagic septicaemia virus (VHSV) infection in rainbow trout (Oncorhynchus mykiss) is greatly increased by combining viral isolation with specific antibody detection",
keywords = "Neutralising antibodies, Rainbow trout, VHSV infection, Serology, Plaque neutralization test",
publisher = "Academic Press",
author = "Schyth, {Brian Dall} and Ellen Ariel and H. Korsholm and Olesen, {Niels Jørgen}",
year = "2012",
doi = "10.1016/j.fsi.2011.12.007",
volume = "32",
number = "4",
pages = "593--597",
journal = "Fish and Shellfish Immunology",
issn = "1050-4648",

}

RIS

TY - JOUR

T1 - Diagnostic capacity for viral haemorrhagic septicaemia virus (VHSV) infection in rainbow trout (Oncorhynchus mykiss) is greatly increased by combining viral isolation with specific antibody detection

A1 - Schyth,Brian Dall

A1 - Ariel,Ellen

A1 - Korsholm,H.

A1 - Olesen,Niels Jørgen

AU - Schyth,Brian Dall

AU - Ariel,Ellen

AU - Korsholm,H.

AU - Olesen,Niels Jørgen

PB - Academic Press

PY - 2012

Y1 - 2012

N2 - Detection of disease specific antibodies in farmed rainbow trout (Oncorhynchus mykiss) has been proposed as an alternative or supplement to the currently approved procedures for diagnosis and surveillance in this species. In samples from natural outbreaks of the disease viral haemorrhagic septicaemia (VHS) at two freshwater farms in southern Denmark serologic testing was used to broaden the diagnostic window from outbreak to diagnosis in the laboratory as compared to traditional procedures of isolation and identification of the virus. The serologic assay clearly increased the chance of detecting present or previous infections where the pathogen could not be isolated by standard methods (indicating older infections where the virus had been cleared). Our data allowed us to monitor the levels of neutralising antibodies in relation to the presence of the virus in fish experiencing two different types of outbreaks at two different farms. By sequence analysis of the viral glycoprotein from selected isolates we found no evidence for escape mutants having developed in the fish showing high titres of neutralising antibodies.

AB - Detection of disease specific antibodies in farmed rainbow trout (Oncorhynchus mykiss) has been proposed as an alternative or supplement to the currently approved procedures for diagnosis and surveillance in this species. In samples from natural outbreaks of the disease viral haemorrhagic septicaemia (VHS) at two freshwater farms in southern Denmark serologic testing was used to broaden the diagnostic window from outbreak to diagnosis in the laboratory as compared to traditional procedures of isolation and identification of the virus. The serologic assay clearly increased the chance of detecting present or previous infections where the pathogen could not be isolated by standard methods (indicating older infections where the virus had been cleared). Our data allowed us to monitor the levels of neutralising antibodies in relation to the presence of the virus in fish experiencing two different types of outbreaks at two different farms. By sequence analysis of the viral glycoprotein from selected isolates we found no evidence for escape mutants having developed in the fish showing high titres of neutralising antibodies.

KW - Neutralising antibodies

KW - Rainbow trout

KW - VHSV infection

KW - Serology

KW - Plaque neutralization test

U2 - 10.1016/j.fsi.2011.12.007

DO - 10.1016/j.fsi.2011.12.007

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

IS - 4

VL - 32

SP - 593

EP - 597

ER -