TY - JOUR

T1 - Development of an Electrochemical Metal-Ion Biosensor Using Self-Assembled Peptide Nanofibrils

A1 - Viguier,Bruno

A1 - Zor,Kinga

A1 - Kasotakis,Emmanouil

A1 - Mitraki,Anna

A1 - Clausen,Casper Hyttel

A1 - Svendsen,Winnie Edith

A1 - Castillo,Jaime

AU - Viguier,Bruno

AU - Zor,Kinga

AU - Kasotakis,Emmanouil

AU - Mitraki,Anna

AU - Clausen,Casper Hyttel

AU - Svendsen,Winnie Edith

AU - Castillo,Jaime

PB - American Chemical Society

PY - 2011

Y1 - 2011

N2 - This article describes the combination of self-assembled peptide nanofibrils with metal electrodes for the development of an electrochemical metal-ion biosensor. The biological nanofibrils were immobilized on gold electrodes and used as biorecognition elements for the complexation with copper ions. These nanofibrils were obtained under aqueous conditions, at room temperature and outside the clean room. The functionalized gold electrode was evaluated by cyclic voltammetry, impedance spectroscopy, energy dispersive X-ray and atomic force microscopy. The obtained results displayed a layer of nanofibrils able to complex with copper ions in solution. The response of the obtained biosensor was linear up to 50 µM copper and presented a sensitivity of 0.68 µAcm-2µM-1. Moreover, the fabricated sensor could be regenerated to a copper-free state allowing its re-utilization.

AB - This article describes the combination of self-assembled peptide nanofibrils with metal electrodes for the development of an electrochemical metal-ion biosensor. The biological nanofibrils were immobilized on gold electrodes and used as biorecognition elements for the complexation with copper ions. These nanofibrils were obtained under aqueous conditions, at room temperature and outside the clean room. The functionalized gold electrode was evaluated by cyclic voltammetry, impedance spectroscopy, energy dispersive X-ray and atomic force microscopy. The obtained results displayed a layer of nanofibrils able to complex with copper ions in solution. The response of the obtained biosensor was linear up to 50 µM copper and presented a sensitivity of 0.68 µAcm-2µM-1. Moreover, the fabricated sensor could be regenerated to a copper-free state allowing its re-utilization.

U2 - 10.1021/am200149h

DO - 10.1021/am200149h

JO - A C S Applied Materials and Interfaces

JF - A C S Applied Materials and Interfaces

SN - 1944-8244

IS - 5

VL - 3

SP - 1594

EP - 1600

ER -