Development of a rapid method for direct detection of tet(M) genes in soil from Danish farmland

Publication: Research - peer-reviewJournal article – Annual report year: 2004

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A method for direct detection of antibiotic resistance genes in soil samples has been developed. The tetracycline resistance gene, tet(M), was used as a model. The method was validated on Danish farmland soil that had repeatedly been treated with pig manure slurry containing resistant bacteria. The tet(M) gene was directly detected in 10-80% of the samples from the various farmland soils and could be detected in all samples tested after selective enrichment. To validate the obtained results, the method was applied to garden soil samples where lower prevalence of resistance was found. Result: A detection limit of 10(2)-10(3) copies of the tet(M) gene per gram of soil (in a Bacillus cereus group bacterium) was achieved. tet(M) gene was detected in soil samples with the highest prevalence on farmland treated with pig manure slurry.
Original languageEnglish
JournalEnvironment International
Publication date2004
Volume30
Issue1
Pages117-122
ISSN0160-4120
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 16

Keywords

  • DNA extraction, direct detection, soil, tet(M)
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