Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa. / Ito, T.; Olesen, Niels Jørgen; Skall, Helle Frank; Sano, M.; Kurita, J.; Nakajima, K.; Iida, T.

In: Diseases of Aquatic Organisms, Vol. 89, No. 1, 2010, p. 17-27.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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Ito, T.; Olesen, Niels Jørgen; Skall, Helle Frank; Sano, M.; Kurita, J.; Nakajima, K.; Iida, T. / Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa.

In: Diseases of Aquatic Organisms, Vol. 89, No. 1, 2010, p. 17-27.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Bibtex

@article{bcbb8c6dd74f44af8c7de70de9c5d157,
title = "Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa",
publisher = "Inter-Research",
author = "T. Ito and Olesen, {Niels Jørgen} and Skall, {Helle Frank} and M. Sano and J. Kurita and K. Nakajima and T. Iida",
year = "2010",
doi = "10.3354/dao02183",
volume = "89",
number = "1",
pages = "17--27",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",

}

RIS

TY - JOUR

T1 - Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa

A1 - Ito,T.

A1 - Olesen,Niels Jørgen

A1 - Skall,Helle Frank

A1 - Sano,M.

A1 - Kurita,J.

A1 - Nakajima,K.

A1 - Iida,T.

AU - Ito,T.

AU - Olesen,Niels Jørgen

AU - Skall,Helle Frank

AU - Sano,M.

AU - Kurita,J.

AU - Nakajima,K.

AU - Iida,T.

PB - Inter-Research

PY - 2010

Y1 - 2010

N2 - The viral haemorrhagic septicaemia virus (VHSV) comprises 4 major genotypes and a number of subtypes with, in most cases, distinct geographical distribution. A quick and simple detection method that can discriminate the different genotypes is desirable for a quick and more efficient prevention of the spread of genotypes to new geographical areas. A monoclonal antibody (MAb) against VHSV genotype IVa was produced, with the aim of providing a simple method of discriminating this genotype from the other VHSV genotypes (I, II, III and IVb). Balb/c mice were injected with purified VHSV-JF00Ehil (genotype IVa) from diseased farmed Japanese flounder. Ten hybridoma clones secreting monoclonal antibodies (MAbs) against VHSV were established. One of these, MAb VHS-10, reacted only with genotype IVa in indirect fluorescent antibody technique (IFAT) and ELISA. Using cell cultures that were transfected with each of the viral protein genes, it was shown that the MAb VHS-10 recognizes a nonlinear genotype IVa-specific epitope on the VHSV N-protein.

AB - The viral haemorrhagic septicaemia virus (VHSV) comprises 4 major genotypes and a number of subtypes with, in most cases, distinct geographical distribution. A quick and simple detection method that can discriminate the different genotypes is desirable for a quick and more efficient prevention of the spread of genotypes to new geographical areas. A monoclonal antibody (MAb) against VHSV genotype IVa was produced, with the aim of providing a simple method of discriminating this genotype from the other VHSV genotypes (I, II, III and IVb). Balb/c mice were injected with purified VHSV-JF00Ehil (genotype IVa) from diseased farmed Japanese flounder. Ten hybridoma clones secreting monoclonal antibodies (MAbs) against VHSV were established. One of these, MAb VHS-10, reacted only with genotype IVa in indirect fluorescent antibody technique (IFAT) and ELISA. Using cell cultures that were transfected with each of the viral protein genes, it was shown that the MAb VHS-10 recognizes a nonlinear genotype IVa-specific epitope on the VHSV N-protein.

U2 - 10.3354/dao02183

DO - 10.3354/dao02183

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 1

VL - 89

SP - 17

EP - 27

ER -