Development of a compact Bio-Optofluidic Cell Sorter
Publication: Research - peer-review › Conference article – Annual report year: 2012
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Development of a compact Bio-Optofluidic Cell Sorter. / Bañas, Andrew Rafael; Palima, Darwin; Pedersen, Finn; Glückstad, Jesper.
In: Proceedings of SPIE, the International Society for Optical Engineering, Vol. 8274, 2012, p. 82740N.Publication: Research - peer-review › Conference article – Annual report year: 2012
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TY - CONF
T1 - Development of a compact Bio-Optofluidic Cell Sorter
A1 - Bañas,Andrew Rafael
A1 - Palima,Darwin
A1 - Pedersen,Finn
A1 - Glückstad,Jesper
AU - Bañas,Andrew Rafael
AU - Palima,Darwin
AU - Pedersen,Finn
AU - Glückstad,Jesper
PB - S P I E - International Society for Optical Engineering
PY - 2012
Y1 - 2012
N2 - We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.
AB - We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.
KW - Optical tweezers or optical manipulation
KW - Machine vision
KW - Spatial light modulators
KW - Image processing
KW - Optical sorting
UR - http://spie.org/x2584.xml
U2 - 10.1117/12.909908
DO - 10.1117/12.909908
JO - Proceedings of SPIE, the International Society for Optical Engineering
JF - Proceedings of SPIE, the International Society for Optical Engineering
SN - 1605-7422
VL - 8274
SP - 82740N
ER -