Development of a compact Bio-Optofluidic Cell Sorter

Publication: Research - peer-reviewConference article – Annual report year: 2012

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@article{e1d425f9f5da489a96ad7cbac80d1b61,
title = "Development of a compact Bio-Optofluidic Cell Sorter",
keywords = "Optical tweezers or optical manipulation, Machine vision, Spatial light modulators, Image processing, Optical sorting",
publisher = "S P I E - International Society for Optical Engineering",
author = "Bañas, {Andrew Rafael} and Darwin Palima and Finn Pedersen and Jesper Glückstad",
year = "2012",
doi = "10.1117/12.909908",
volume = "8274",
pages = "82740N",
journal = "Proceedings of SPIE, the International Society for Optical Engineering",
issn = "1605-7422",

}

RIS

TY - CONF

T1 - Development of a compact Bio-Optofluidic Cell Sorter

A1 - Bañas,Andrew Rafael

A1 - Palima,Darwin

A1 - Pedersen,Finn

A1 - Glückstad,Jesper

AU - Bañas,Andrew Rafael

AU - Palima,Darwin

AU - Pedersen,Finn

AU - Glückstad,Jesper

PB - S P I E - International Society for Optical Engineering

PY - 2012

Y1 - 2012

N2 - We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.

AB - We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.

KW - Optical tweezers or optical manipulation

KW - Machine vision

KW - Spatial light modulators

KW - Image processing

KW - Optical sorting

UR - http://spie.org/x2584.xml

U2 - 10.1117/12.909908

DO - 10.1117/12.909908

JO - Proceedings of SPIE, the International Society for Optical Engineering

JF - Proceedings of SPIE, the International Society for Optical Engineering

SN - 1605-7422

VL - 8274

SP - 82740N

ER -