Development of a compact Bio-Optofluidic Cell Sorter

Publication: Research - peer-reviewConference article – Annual report year: 2012

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We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.
Original languageEnglish
JournalProceedings of SPIE, the International Society for Optical Engineering
Publication date2012
Volume8274
Pages82740N
ISSN0277-786X
DOIs
StatePublished

Conference

ConferenceSPIE Photonics West
CountryUnited States
CitySan Francisco, California
Period21/01/1226/01/12
CitationsWeb of Science® Times Cited: 0

Keywords

  • Optical tweezers or optical manipulation, Machine vision, Spatial light modulators, Image processing, Optical sorting
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