An eradication project was applied in three Danish sheep farms affected with footrot. Methods to detect Dichelobacter nodosus by culture and real-time PCR were established at the National Veterinary Institute and the organism was detected for the first time in Denmark. All sheep were examined twice and all hoofs assessed using a 5-point scale. The prevalence of affected sheep was 24 %, 64 % and 29 % in herd 1, 2 and 3, respectively. Among sheep with characteristic, undermined, foul-smelling lesions (hoof-score ≥3) 96 % were positive for D. nodosus in the real-time PCR. All sheep were treated with 10 % zink-sulphate hoofbath, and oxytetracycline i.m. After treatment only one sheep had a hoof score ≥2. One year after the eradication project only one herd, which has implemented quarantine restrictions, is still PCR negative and free of clinical signs.