TY - JOUR

T1 - Detection of Campylobacter Bacteria in Air Samples for Continuous Real-Time Monitoring of Campylobacter Colonization in Broiler Flocks

A1 - Olsen,Katja Nyholm

A1 - Lund,Marianne

A1 - Skov,J.

A1 - Christensen,Laurids Siig

A1 - Hoorfar,Jeffrey

AU - Olsen,Katja Nyholm

AU - Lund,Marianne

AU - Skov,J.

AU - Christensen,Laurids Siig

AU - Hoorfar,Jeffrey

PB - American Society for Microbiology

PY - 2009

Y1 - 2009

N2 - Improved monitoring tools are important for the control of Campylobacter bacteria in broiler production. In this study, we compare the sensitivities of detection of Campylobacter by PCR with feces, dust, and air samples during the lifetimes of broilers in two poultry houses and conclude that the sensitivity of detection of Campylobacter in air is comparable to that in other sample materials. Profiling of airborne particles in six poultry houses revealed that the aerodynamic conditions were dependent on the age of the chickens and very comparable among different poultry houses, with low proportions of particles in the 0.5- to 2-mu m-diameter range and high proportions in the 2- to 5-mu m-diameter range. Campylobacter could also be detected by PCR in air samples collected at the hanging stage during the slaughter process but not at the other stages tested at the slaughterhouse. The exploitation of airborne dust in poultry houses as a sample material for the detection of Campylobacter and other pathogens provides an intriguing possibility, in conjunction with new detection technologies, for allowing continuous or semicontinuous monitoring of colonization status.

AB - Improved monitoring tools are important for the control of Campylobacter bacteria in broiler production. In this study, we compare the sensitivities of detection of Campylobacter by PCR with feces, dust, and air samples during the lifetimes of broilers in two poultry houses and conclude that the sensitivity of detection of Campylobacter in air is comparable to that in other sample materials. Profiling of airborne particles in six poultry houses revealed that the aerodynamic conditions were dependent on the age of the chickens and very comparable among different poultry houses, with low proportions of particles in the 0.5- to 2-mu m-diameter range and high proportions in the 2- to 5-mu m-diameter range. Campylobacter could also be detected by PCR in air samples collected at the hanging stage during the slaughter process but not at the other stages tested at the slaughterhouse. The exploitation of airborne dust in poultry houses as a sample material for the detection of Campylobacter and other pathogens provides an intriguing possibility, in conjunction with new detection technologies, for allowing continuous or semicontinuous monitoring of colonization status.

U2 - 10.1128/AEM.02182-08

DO - 10.1128/AEM.02182-08

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 7

VL - 75

SP - 2074

EP - 2078

ER -