Detection of biologically active diterpenoic acids by Raman Spectroscopy

Publication: Research - peer-reviewJournal article – Annual report year: 2010

  • Author: Talian, Ivan

    Comenius University, Faculty of Sciences

  • Author: Orinak, Andrej

    P. J. Šafárik University, Faculty of Science

  • Author: Efremov, Evtim V.

    MAPPER Litography, Computerlaan 15

  • Author: Ariese, Freek

    Laser Centre Vrije Universiteit, Department of Analytical Chemistry and Applied Spectroscopy

  • Author: Kaniansky, Dušan

    Comenius University, Faculty of Sciences

  • Author: Orinakova, Renáta

    P. J. Šafárik University, Faculty of Science

  • Author: Hübner, Jörg

    DTU Danchip, Technical University of Denmark, Ørsteds Plads, 2800, Lyngby, Denmark

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Three poorly detectable, biologically active diterpenoic acids, kaurenoic, abietic, and gibberellic acid, were studied by using different modes of Raman spectroscopy. Because of their structural similarities, in the absence of strongly polarizable groups, conventional Raman spectroscopy is not suitable for their unambiguous identification, especially not in solution. We attempted to increase the sensitivity by applying UV-resonance Raman spectroscopy and surface-enhanced Raman spectroscopy (SERS) techniques. The UV-Raman spectra of the three compounds in ethanol/water 50 : 50 showed only very few enhanced Raman lines. SERS spectra with 514-nm excitation with Ag colloids were also relatively weak. The best SERS spectrawere obtained with 785-nm excitation on a novel nanostructured substrate, 'black silicon' coated with a 400-nm gold layer. The spectra showed clear differences, and these 'fingerprints' would be suitable for the unambiguous identification of these diterpenoic acids.
Original languageEnglish
JournalJournal of Raman Spectroscopy
Publication date2010
Volume41
Issue9
Pages964-968
ISSN0377-0486
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 4

Keywords

  • nanostructured substrate, resonance Raman spectroscopy, black silicon, SERS, diterpenoic acids
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