Detection of bacteriophage-infected cells of Lactococcus lactis using flow cytometry

Publication: Research - peer-reviewJournal article – Annual report year: 2007

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Detection of bacteriophage-infected cells of Lactococcus lactis using flow cytometry. / Michelsen, Ole; Cuesta-Dominguez, Álvaro; Albrektsen, Bjarne; Jensen, Peter Ruhdal.

In: Applied and Environmental Microbiology, Vol. 73, No. 23, 2007, p. 7575-81.

Publication: Research - peer-reviewJournal article – Annual report year: 2007

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Author

Michelsen, Ole; Cuesta-Dominguez, Álvaro; Albrektsen, Bjarne; Jensen, Peter Ruhdal / Detection of bacteriophage-infected cells of Lactococcus lactis using flow cytometry.

In: Applied and Environmental Microbiology, Vol. 73, No. 23, 2007, p. 7575-81.

Publication: Research - peer-reviewJournal article – Annual report year: 2007

Bibtex

@article{e60d77a3adf14dc7aef283485fcca311,
title = "Detection of bacteriophage-infected cells of Lactococcus lactis using flow cytometry",
publisher = "American Society for Microbiology",
author = "Ole Michelsen and Álvaro Cuesta-Dominguez and Bjarne Albrektsen and Jensen, {Peter Ruhdal}",
year = "2007",
doi = "10.1128/AEM.01219-07",
volume = "73",
number = "23",
pages = "7575--81",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",

}

RIS

TY - JOUR

T1 - Detection of bacteriophage-infected cells of Lactococcus lactis using flow cytometry

A1 - Michelsen,Ole

A1 - Cuesta-Dominguez,Álvaro

A1 - Albrektsen,Bjarne

A1 - Jensen,Peter Ruhdal

AU - Michelsen,Ole

AU - Cuesta-Dominguez,Álvaro

AU - Albrektsen,Bjarne

AU - Jensen,Peter Ruhdal

PB - American Society for Microbiology

PY - 2007

Y1 - 2007

N2 - Bacteriophage infection in dairy fermentation constitutes a serious problem worldwide. We have studied bacteriophage infection in Lactococcus lactis by using the flow cytometer. The first effect of the infection of the bacterium is a change from cells in chains toward single cells. We interpret this change as a consequence of a cease in cell growth, while the ongoing cell divisions leave the cells as single cells. Late in the infection cycle, cells with low-density cell walls appear, and these cells can be detected on cytograms of light scatter versus, for instance, fluorescence of stained DNA. We describe a new method for detection of phage infection in Lactococcus lactis dairy cultures. The method is based on flow cytometric detection of cells with low-density cell walls. The method allows fast and early detection of phage-infected bacteria, independently of which phage has infected the culture. The method can be performed in real time and therefore increases the chance of successful intervention in the fermentation process.

AB - Bacteriophage infection in dairy fermentation constitutes a serious problem worldwide. We have studied bacteriophage infection in Lactococcus lactis by using the flow cytometer. The first effect of the infection of the bacterium is a change from cells in chains toward single cells. We interpret this change as a consequence of a cease in cell growth, while the ongoing cell divisions leave the cells as single cells. Late in the infection cycle, cells with low-density cell walls appear, and these cells can be detected on cytograms of light scatter versus, for instance, fluorescence of stained DNA. We describe a new method for detection of phage infection in Lactococcus lactis dairy cultures. The method is based on flow cytometric detection of cells with low-density cell walls. The method allows fast and early detection of phage-infected bacteria, independently of which phage has infected the culture. The method can be performed in real time and therefore increases the chance of successful intervention in the fermentation process.

U2 - 10.1128/AEM.01219-07

DO - 10.1128/AEM.01219-07

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 23

VL - 73

SP - 7575

EP - 7581

ER -