Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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@article{bf3fa168ef1f451d83cf737e39b2269c,
title = "Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp.",
keywords = "Brown seaweed, Extraction method, Sargassum, Bioactive compound, Fucose, Fucoidan",
publisher = "American Physiological Society",
author = "Ale, {Marcel Tutor} and Mikkelsen, {Jørn Dalgaard} and Meyer, {Anne S.}",
year = "2012",
doi = "10.1007/s10811-011-9690-3",
volume = "24",
number = "4",
pages = "715--723",
journal = "Journal of Applied Physiology",
issn = "8750-7587",

}

RIS

TY - JOUR

T1 - Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp.

A1 - Ale,Marcel Tutor

A1 - Mikkelsen,Jørn Dalgaard

A1 - Meyer,Anne S.

AU - Ale,Marcel Tutor

AU - Mikkelsen,Jørn Dalgaard

AU - Meyer,Anne S.

PB - American Physiological Society

PY - 2012

Y1 - 2012

N2 - Fucose-containing sulfated polysaccharides can be extracted from the brown seaweed, Sargassum sp. It has been reported that fucose-rich sulfated polysaccharides from brown seaweeds exert different beneficial biological activities including anti-inflammatory, anticoagulant, and anti-viral effects. Classical extraction of fucose-containing sulfated polysaccharides from brown seaweed species typically involves extended, multiple-step, hot acid, or CaCl2 treatments, each step lasting several hours. In this work, we systematically examined the influence of acid concentration (HCl), time, and temperature on the yield of fucosecontaining sulfated polysaccharides (FCSPs) in statistically designed two-step and single-step multifactorial extraction experiments. All extraction factors had significant effects on the fucose-containing sulfated polysaccharides yield, with the temperature and time exerting positive effects, and the acid concentration having a negative effect. The model defined an optimized single-step FCSPs extraction procedure for Sargassum sp. (a brown seaweed). A maximal fucosecontaining sulfated polysaccharides yield of ∼7% of the Sargassum sp. dry matter was achieved by the optimal extraction procedure of: 0.03 M HCl, 90°C, 4 h. HPAECPAD analysis confirmed that fucose, galactose, and glucuronic acid were the major constituents of the polysaccharides obtained by the optimized method. Lower polysaccharide yield, but relatively higher fucose content was obtained with shorter extraction time. The data also revealed that classical multi-step extraction with acid ≥0.2 M HCl at elevated temperature and extended time had a detrimental effect on the FCSPs yield as this treatment apparently disrupted the structural integrity of the polymer and evidently caused degradation of the carbohydrate chains built up of fucose residues.

AB - Fucose-containing sulfated polysaccharides can be extracted from the brown seaweed, Sargassum sp. It has been reported that fucose-rich sulfated polysaccharides from brown seaweeds exert different beneficial biological activities including anti-inflammatory, anticoagulant, and anti-viral effects. Classical extraction of fucose-containing sulfated polysaccharides from brown seaweed species typically involves extended, multiple-step, hot acid, or CaCl2 treatments, each step lasting several hours. In this work, we systematically examined the influence of acid concentration (HCl), time, and temperature on the yield of fucosecontaining sulfated polysaccharides (FCSPs) in statistically designed two-step and single-step multifactorial extraction experiments. All extraction factors had significant effects on the fucose-containing sulfated polysaccharides yield, with the temperature and time exerting positive effects, and the acid concentration having a negative effect. The model defined an optimized single-step FCSPs extraction procedure for Sargassum sp. (a brown seaweed). A maximal fucosecontaining sulfated polysaccharides yield of ∼7% of the Sargassum sp. dry matter was achieved by the optimal extraction procedure of: 0.03 M HCl, 90°C, 4 h. HPAECPAD analysis confirmed that fucose, galactose, and glucuronic acid were the major constituents of the polysaccharides obtained by the optimized method. Lower polysaccharide yield, but relatively higher fucose content was obtained with shorter extraction time. The data also revealed that classical multi-step extraction with acid ≥0.2 M HCl at elevated temperature and extended time had a detrimental effect on the FCSPs yield as this treatment apparently disrupted the structural integrity of the polymer and evidently caused degradation of the carbohydrate chains built up of fucose residues.

KW - Brown seaweed

KW - Extraction method

KW - Sargassum

KW - Bioactive compound

KW - Fucose

KW - Fucoidan

U2 - 10.1007/s10811-011-9690-3

DO - 10.1007/s10811-011-9690-3

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 4

VL - 24

SP - 715

EP - 723

ER -