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Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment. / Vermeiren, Joan; Van den Abbeele, Pieter; Laukens, Debby; Vigsnæs, Louise Kristine; De Vos, Martine; Boon, Nico; Van de Wiele, Tom.

In: F E M S Microbiology Ecology, Vol. 79, No. 3, 2012, p. 685-696.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Vermeiren, Joan; Van den Abbeele, Pieter; Laukens, Debby; Vigsnæs, Louise Kristine; De Vos, Martine; Boon, Nico; Van de Wiele, Tom / Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment.

In: F E M S Microbiology Ecology, Vol. 79, No. 3, 2012, p. 685-696.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{3efd6ad5d93745148522779ab9d936c7,
title = "Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment",
keywords = "Inflammatory bowel disease, Clostridium, Dysbiosis, SHIME, Adhesion, MAMC",
publisher = "Wiley-Blackwell Publishing Ltd.",
author = "Joan Vermeiren and {Van den Abbeele}, Pieter and Debby Laukens and Vigsnæs, {Louise Kristine} and {De Vos}, Martine and Nico Boon and {Van de Wiele}, Tom",
year = "2012",
doi = "10.1111/j.1574-6941.2011.01252.x",
volume = "79",
number = "3",
pages = "685--696",
journal = "F E M S Microbiology Ecology",
issn = "0168-6496",

}

RIS

TY - JOUR

T1 - Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment

A1 - Vermeiren,Joan

A1 - Van den Abbeele,Pieter

A1 - Laukens,Debby

A1 - Vigsnæs,Louise Kristine

A1 - De Vos,Martine

A1 - Boon,Nico

A1 - Van de Wiele,Tom

AU - Vermeiren,Joan

AU - Van den Abbeele,Pieter

AU - Laukens,Debby

AU - Vigsnæs,Louise Kristine

AU - De Vos,Martine

AU - Boon,Nico

AU - Van de Wiele,Tom

PB - Wiley-Blackwell Publishing Ltd.

PY - 2012

Y1 - 2012

N2 - The mucus layer in the colon, acting as a barrier to prevent invasion of pathogens, is thinner and discontinuous in patients with ulcerative colitis (UC). A recent developed in vitro dynamic gut model, the M-SHIME, was used to compare long-term colonization of the mucin layer by the microbiota from six healthy volunteers (HV) and six UC patients and thus distinguish the mucin adhered from the luminal microbiota. Although under the same nutritional conditions, short-chain fatty acid production by the luminal communities from UC patients showed a tendency toward a lower butyrate production. A more in-depth community analysis of those microbial groups known to produce butyrate revealed that the diversity of the Clostridium coccoides/Eubacterium rectale and Clostridium leptum group, and counts of Faecalibacterium prausnitzii were lower in the luminal fractions of the UC samples. Counts of Roseburia spp. were lower in the mucosal fractions of the UC samples. qPCR analysis for butyryl-CoA:acetate CoA transferase, responsible for butyrate production, displayed a lower abundance in both the luminal and mucosal fractions of the UC samples. The M-SHIME model revealed depletion in butyrate producing microbial communities not restricted to the luminal but also in the mucosal samples from UC patients compared to HV.

AB - The mucus layer in the colon, acting as a barrier to prevent invasion of pathogens, is thinner and discontinuous in patients with ulcerative colitis (UC). A recent developed in vitro dynamic gut model, the M-SHIME, was used to compare long-term colonization of the mucin layer by the microbiota from six healthy volunteers (HV) and six UC patients and thus distinguish the mucin adhered from the luminal microbiota. Although under the same nutritional conditions, short-chain fatty acid production by the luminal communities from UC patients showed a tendency toward a lower butyrate production. A more in-depth community analysis of those microbial groups known to produce butyrate revealed that the diversity of the Clostridium coccoides/Eubacterium rectale and Clostridium leptum group, and counts of Faecalibacterium prausnitzii were lower in the luminal fractions of the UC samples. Counts of Roseburia spp. were lower in the mucosal fractions of the UC samples. qPCR analysis for butyryl-CoA:acetate CoA transferase, responsible for butyrate production, displayed a lower abundance in both the luminal and mucosal fractions of the UC samples. The M-SHIME model revealed depletion in butyrate producing microbial communities not restricted to the luminal but also in the mucosal samples from UC patients compared to HV.

KW - Inflammatory bowel disease

KW - Clostridium

KW - Dysbiosis

KW - SHIME

KW - Adhesion

KW - MAMC

U2 - 10.1111/j.1574-6941.2011.01252.x

DO - 10.1111/j.1574-6941.2011.01252.x

JO - F E M S Microbiology Ecology

JF - F E M S Microbiology Ecology

SN - 0168-6496

IS - 3

VL - 79

SP - 685

EP - 696

ER -