Curvature of Synthetic and Natural Surfaces Is an Important Target Feature in Classical Pathway Complement Activation

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Without internal affiliation

  • Author: Pedersen, Martin Bjerregård

    Aarhus University

  • Author: Zhou, Xingfei

    Aarhus University

  • Author: Larsen, Esben Kjær Unmack

    Unknown

  • Author: Sørensen, Uffe Skov

    Aarhus University

  • Author: Kjems, Jørgen

    Aarhus University

  • Author: Nygaard, Jens Vinge

    Aarhus University

  • Author: Nyengaard, Jens Randel

    Aarhus University

  • Author: Meyer, Rikke Louise

    Aarhus University

  • Author: Boesen, Thomas

    Aarhus University

  • Author: Vorup-Jensen, Thomas

    Aarhus University

View graph of relations

Thebinding ofAbs to microbial surfaces followed by complement activation constitutes animportant line of defense against infections. In this study, we have investigated the relationship between complement activation and the binding ofhuman IgMAbs to surfaces with different curvatures. IgM Abs to dextran were shown to activate complement potently on dextran-coated particles having a diameter around 250 nm, whereas larger (600 nm) particles were less potent activators. This selectivity regarding particle dimension was also found for complement activation by colloidal substances of microbial origin.Peptidoglycan (PGN) is the major chemical component in the cell wall of Gram-positive bacteria. Fragments of purifiedPGNwith sizes of∼100nmpromoted complement activation effectively through the classical pathway. By contrast, larger or smaller fragments of PGN did not activate complement strongly. A careful analysis of PGN fragments released during planctonic growth of Staphylococcus aureus showed that these include curvatures that would permit strong IgM-mediated complement activation, whereas the curvature of intact cells would be less effective for such activation. Consistently, we found that the suspended PGN fragments were strong activators of complement through the classical pathway.We suggest that these fragments act as decoy targets for complement activation, providing protection for S. aureus against the host immune response to infection.
Original languageEnglish
JournalJournal of Immunology
Publication date2010
Volume184
Pages1931-1945
ISSN0022-1767
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 25
Download as:
Download as PDF
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
PDF
Download as HTML
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
HTML
Download as Word
Select render style:
APAAuthorCBEHarvardMLAStandardVancouverShortLong
Word

ID: 5650705