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Comparison of the performance of five different immunoassays to detect specific antibodies against emerging atypical bovine pestivirus. / Larska, Magdalena; Polak, Mirosław P.; Liu, Lihong ; Alenius, Stefan; Uttenthal, Åse.

In: Journal of Virological Methods, Vol. 187, No. 1, 2013, p. 103-109.

Publication: Research - peer-reviewJournal article – Annual report year: 2013

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Author

Larska, Magdalena; Polak, Mirosław P.; Liu, Lihong ; Alenius, Stefan; Uttenthal, Åse / Comparison of the performance of five different immunoassays to detect specific antibodies against emerging atypical bovine pestivirus.

In: Journal of Virological Methods, Vol. 187, No. 1, 2013, p. 103-109.

Publication: Research - peer-reviewJournal article – Annual report year: 2013

Bibtex

@article{82d4fa9ca70b46a5b7e1ade08a5f0323,
title = "Comparison of the performance of five different immunoassays to detect specific antibodies against emerging atypical bovine pestivirus",
publisher = "Elsevier BV",
author = "Magdalena Larska and Polak, {Mirosław P.} and Lihong Liu and Stefan Alenius and Åse Uttenthal",
year = "2013",
doi = "10.1016/j.jviromet.2012.09.010",
volume = "187",
number = "1",
pages = "103--109",
journal = "Journal of Virological Methods",
issn = "0166-0934",

}

RIS

TY - JOUR

T1 - Comparison of the performance of five different immunoassays to detect specific antibodies against emerging atypical bovine pestivirus

A1 - Larska,Magdalena

A1 - Polak,Mirosław P.

A1 - Liu,Lihong

A1 - Alenius,Stefan

A1 - Uttenthal,Åse

AU - Larska,Magdalena

AU - Polak,Mirosław P.

AU - Liu,Lihong

AU - Alenius,Stefan

AU - Uttenthal,Åse

PB - Elsevier BV

PY - 2013

Y1 - 2013

N2 - Bovine pestiviruses represent a considerably variable group. In addition to the two accepted species BVDV-1 and BVDV-2, a number of atypical bovine pestiviruses have been detected both in foetal calf sera and in field samples. The sera collected during the initial six weeks of experimental infection of calves with atypical pestivirus, BVDV-1 and a combination of both viruses have been examined by routine and new diagnostic tests to validate their robustness and sensitivity. As expected, virus neutralization tests using homologous virus were able to differentiate the two groups infected by BVDV-1 or atypical pestivirus, whereas the animals inoculated with a mixture of these two viruses had a reaction pattern very similar to the homologous virus alone. It was found that immunoassays using whole virus and polyclonal antibodies are the most robust, but all tests examined were able to detect antibodies also from cattle infected with atypical pestivirus a few weeks after infection. The detection, however, was at a lower level and slightly delayed. Statistical validation of the threshold suggested by the manufacturer showed that in some cases the reduction of the cut-off values would improve the test sensitivity.

AB - Bovine pestiviruses represent a considerably variable group. In addition to the two accepted species BVDV-1 and BVDV-2, a number of atypical bovine pestiviruses have been detected both in foetal calf sera and in field samples. The sera collected during the initial six weeks of experimental infection of calves with atypical pestivirus, BVDV-1 and a combination of both viruses have been examined by routine and new diagnostic tests to validate their robustness and sensitivity. As expected, virus neutralization tests using homologous virus were able to differentiate the two groups infected by BVDV-1 or atypical pestivirus, whereas the animals inoculated with a mixture of these two viruses had a reaction pattern very similar to the homologous virus alone. It was found that immunoassays using whole virus and polyclonal antibodies are the most robust, but all tests examined were able to detect antibodies also from cattle infected with atypical pestivirus a few weeks after infection. The detection, however, was at a lower level and slightly delayed. Statistical validation of the threshold suggested by the manufacturer showed that in some cases the reduction of the cut-off values would improve the test sensitivity.

KW - Atypical bovine pestivirus

KW - Enzyme-linked immunosorbent assay

KW - (ELISA)

KW - Microsphere immunoassay (MIA)

KW - Diagnosis

KW - Antibody detection

U2 - 10.1016/j.jviromet.2012.09.010

DO - 10.1016/j.jviromet.2012.09.010

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 1

VL - 187

SP - 103

EP - 109

ER -