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Comparison of different concentration methods for the detection of hepatitis A virus and calicivirus from bottled natural mineral waters. / Di Pasquale, S.; Paniconi, M; Auricchio, B; Orefice, L; Schultz, Anna Charlotte; De Medici, D.

In: Journal of Virological Methods, Vol. 165, No. 1, 2010, p. 57-63.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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Author

Di Pasquale, S.; Paniconi, M; Auricchio, B; Orefice, L; Schultz, Anna Charlotte; De Medici, D / Comparison of different concentration methods for the detection of hepatitis A virus and calicivirus from bottled natural mineral waters.

In: Journal of Virological Methods, Vol. 165, No. 1, 2010, p. 57-63.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Bibtex

@article{290084ca2e6640c8875ebb887297a42f,
title = "Comparison of different concentration methods for the detection of hepatitis A virus and calicivirus from bottled natural mineral waters",
publisher = "Elsevier BV",
author = "{Di Pasquale}, S. and M Paniconi and B Auricchio and L Orefice and Schultz, {Anna Charlotte} and {De Medici}, D",
year = "2010",
doi = "10.1016/j.jviromet.2010.01.003",
volume = "165",
number = "1",
pages = "57--63",
journal = "Journal of Virological Methods",
issn = "0166-0934",

}

RIS

TY - JOUR

T1 - Comparison of different concentration methods for the detection of hepatitis A virus and calicivirus from bottled natural mineral waters

A1 - Di Pasquale,S.

A1 - Paniconi,M

A1 - Auricchio,B

A1 - Orefice,L

A1 - Schultz,Anna Charlotte

A1 - De Medici,D

AU - Di Pasquale,S.

AU - Paniconi,M

AU - Auricchio,B

AU - Orefice,L

AU - Schultz,Anna Charlotte

AU - De Medici,D

PB - Elsevier BV

PY - 2010

Y1 - 2010

N2 - To evaluate the efficiency of different recovery methods of viral RNA from bottled water, a comparison was made of 2 positively and 2 negatively charged membranes that were used for absorbing and releasing HAV virus particles during the filtration of viral spiked bottled water. All the 4 membranes, regardless of charge and pore size, had low level viral recovery. The results show that a considerable number of the virus particles passed through the pores of the membranes instead of being trapped by the electrostatic charges. Two different procedures were then compared using 1.5 L polyethylene bottles spiked with 10-fold serial dilutions of HAV and FCV. The first procedure included an ultrafiltration-based method followed by MiniMag RNA extraction, and the second an ultracentrifugation-based method followed by RNA extraction using QIAamp® viral RNA mini kit. The ultracentrifugation-based method resulted in a better recovery of HAV and FCV when compared to the ultrafiltration-based method.

AB - To evaluate the efficiency of different recovery methods of viral RNA from bottled water, a comparison was made of 2 positively and 2 negatively charged membranes that were used for absorbing and releasing HAV virus particles during the filtration of viral spiked bottled water. All the 4 membranes, regardless of charge and pore size, had low level viral recovery. The results show that a considerable number of the virus particles passed through the pores of the membranes instead of being trapped by the electrostatic charges. Two different procedures were then compared using 1.5 L polyethylene bottles spiked with 10-fold serial dilutions of HAV and FCV. The first procedure included an ultrafiltration-based method followed by MiniMag RNA extraction, and the second an ultracentrifugation-based method followed by RNA extraction using QIAamp® viral RNA mini kit. The ultracentrifugation-based method resulted in a better recovery of HAV and FCV when compared to the ultrafiltration-based method.

KW - Ultrafiltration

KW - Concentration methods

KW - Bottled water

KW - Calicivirus

KW - Hepatitis A

KW - Ultracentrifugation

KW - Real Time-PCR

U2 - 10.1016/j.jviromet.2010.01.003

DO - 10.1016/j.jviromet.2010.01.003

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 1

VL - 165

SP - 57

EP - 63

ER -