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To evaluate the efficiency of different recovery methods of viral RNA from bottled water, a comparison was made of 2 positively and 2 negatively charged membranes that were used for absorbing and releasing HAV virus particles during the filtration of viral spiked bottled water. All the 4 membranes, regardless of charge and pore size, had low level viral recovery. The results show that a considerable number of the virus particles passed through the pores of the membranes instead of being trapped by the electrostatic charges. Two different procedures were then compared using 1.5 L polyethylene bottles spiked with 10-fold serial dilutions of HAV and FCV. The first procedure included an ultrafiltration-based method followed by MiniMag RNA extraction, and the second an ultracentrifugation-based method followed by RNA extraction using QIAamp® viral RNA mini kit. The ultracentrifugation-based method resulted in a better recovery of HAV and FCV when compared to the ultrafiltration-based method.
Original languageEnglish
JournalJournal of Virological Methods
Publication date2010
Volume165
Issue1
Pages57-63
ISSN0166-0934
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 14

Keywords

  • Ultrafiltration, Concentration methods, Bottled water, Calicivirus, Hepatitis A, Ultracentrifugation, Real Time-PCR
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