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Comparative analysis of inflamed and non-inflamed colon biopsies reveals strong proteomic inflammation profile in patients with ulcerative colitis. / Poulsen, Nina Aagaard; Andersen, Vibeke; Moller, Jens Christian; Møller, Hanne S.; Jessen, Flemming; Purup, Stig; Larsen, Lotte B.

In: B M C Gastroenterology, Vol. 12, No. 76, 2012.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Poulsen, Nina Aagaard; Andersen, Vibeke; Moller, Jens Christian; Møller, Hanne S.; Jessen, Flemming; Purup, Stig; Larsen, Lotte B. / Comparative analysis of inflamed and non-inflamed colon biopsies reveals strong proteomic inflammation profile in patients with ulcerative colitis.

In: B M C Gastroenterology, Vol. 12, No. 76, 2012.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{716f62ebe5014c53b9f2621ba8585477,
title = "Comparative analysis of inflamed and non-inflamed colon biopsies reveals strong proteomic inflammation profile in patients with ulcerative colitis",
publisher = "BioMed Central Ltd.",
author = "Poulsen, {Nina Aagaard} and Vibeke Andersen and Moller, {Jens Christian} and Møller, {Hanne S.} and Flemming Jessen and Stig Purup and Larsen, {Lotte B.}",
year = "2012",
doi = "10.1186/1471-230X-12-76",
volume = "12",
number = "76",
journal = "B M C Gastroenterology",
issn = "1471-230X",

}

RIS

TY - JOUR

T1 - Comparative analysis of inflamed and non-inflamed colon biopsies reveals strong proteomic inflammation profile in patients with ulcerative colitis

A1 - Poulsen,Nina Aagaard

A1 - Andersen,Vibeke

A1 - Moller,Jens Christian

A1 - Møller,Hanne S.

A1 - Jessen,Flemming

A1 - Purup,Stig

A1 - Larsen,Lotte B.

AU - Poulsen,Nina Aagaard

AU - Andersen,Vibeke

AU - Moller,Jens Christian

AU - Møller,Hanne S.

AU - Jessen,Flemming

AU - Purup,Stig

AU - Larsen,Lotte B.

PB - BioMed Central Ltd.

PY - 2012

Y1 - 2012

N2 - Background: Accurate diagnostic and monitoring tools for ulcerative colitis (UC) are missing. Our aim was to describe the proteomic profile of UC and search for markers associated with disease exacerbation. Therefore, we aimed to characterize specific proteins associated with inflamed colon mucosa from patients with acute UC using mass spectrometry-based proteomic analysis. Methods: Biopsies were sampled from rectum, sigmoid colon and left colonic flexure from twenty patients with active proctosigmoiditis and from four healthy controls for proteomics and histology. Proteomic profiles of whole colonic biopsies were characterized using 2D-gel electrophoresis, and peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for identification of differently expressed protein spots. Results: A total of 597 spots were annotated by image analysis and 222 of these had a statistically different protein level between inflamed and non-inflamed tissue in the patient group. Principal component analysis clearly grouped non-inflamed samples separately from the inflamed samples indicating that the proteomic signature of colon mucosa with acute UC is strong. Totally, 43 individual protein spots were identified, including proteins involved in energy metabolism (triosephosphate isomerase, glycerol-3-phosphate-dehydrogenase, alpha enolase and L-lactate dehydrogenase B-chain) and in oxidative stress (superoxide dismutase, thioredoxins and selenium binding protein). Conclusions: A distinct proteomic profile of inflamed tissue in UC patients was found. Specific proteins involved in energy metabolism and oxidative stress were identified as potential candidate markers for UC.

AB - Background: Accurate diagnostic and monitoring tools for ulcerative colitis (UC) are missing. Our aim was to describe the proteomic profile of UC and search for markers associated with disease exacerbation. Therefore, we aimed to characterize specific proteins associated with inflamed colon mucosa from patients with acute UC using mass spectrometry-based proteomic analysis. Methods: Biopsies were sampled from rectum, sigmoid colon and left colonic flexure from twenty patients with active proctosigmoiditis and from four healthy controls for proteomics and histology. Proteomic profiles of whole colonic biopsies were characterized using 2D-gel electrophoresis, and peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for identification of differently expressed protein spots. Results: A total of 597 spots were annotated by image analysis and 222 of these had a statistically different protein level between inflamed and non-inflamed tissue in the patient group. Principal component analysis clearly grouped non-inflamed samples separately from the inflamed samples indicating that the proteomic signature of colon mucosa with acute UC is strong. Totally, 43 individual protein spots were identified, including proteins involved in energy metabolism (triosephosphate isomerase, glycerol-3-phosphate-dehydrogenase, alpha enolase and L-lactate dehydrogenase B-chain) and in oxidative stress (superoxide dismutase, thioredoxins and selenium binding protein). Conclusions: A distinct proteomic profile of inflamed tissue in UC patients was found. Specific proteins involved in energy metabolism and oxidative stress were identified as potential candidate markers for UC.

UR - http://www.biomedcentral.com.globalproxy.cvt.dk/1471-230X/12/76

U2 - 10.1186/1471-230X-12-76

DO - 10.1186/1471-230X-12-76

JO - B M C Gastroenterology

JF - B M C Gastroenterology

SN - 1471-230X

IS - 76

VL - 12

ER -