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  • Author: Poulsen, Nina Aagaard

    Aarhus University, Denmark

  • Author: Andersen, Vibeke

    Medical Department, Viborg Regional Hospital, Denmark

  • Author: Moller, Jens Christian

    Pathological Department, Viborg Regional Hospital, Denmark

  • Author: Møller, Hanne S.

    Aarhus University, Denmark

  • Author: Jessen, Flemming

    Division of Industrial Food Research, National Food Institute, Technical University of Denmark, Søltofts Plads, 2800, Kgs. Lyngby, Denmark

  • Author: Purup, Stig

    Aarhus University, Denmark

  • Author: Larsen, Lotte B.

    Aarhus University, Denmark

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Background: Accurate diagnostic and monitoring tools for ulcerative colitis (UC) are missing. Our aim was to describe the proteomic profile of UC and search for markers associated with disease exacerbation. Therefore, we aimed to characterize specific proteins associated with inflamed colon mucosa from patients with acute UC using mass spectrometry-based proteomic analysis. Methods: Biopsies were sampled from rectum, sigmoid colon and left colonic flexure from twenty patients with active proctosigmoiditis and from four healthy controls for proteomics and histology. Proteomic profiles of whole colonic biopsies were characterized using 2D-gel electrophoresis, and peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was applied for identification of differently expressed protein spots. Results: A total of 597 spots were annotated by image analysis and 222 of these had a statistically different protein level between inflamed and non-inflamed tissue in the patient group. Principal component analysis clearly grouped non-inflamed samples separately from the inflamed samples indicating that the proteomic signature of colon mucosa with acute UC is strong. Totally, 43 individual protein spots were identified, including proteins involved in energy metabolism (triosephosphate isomerase, glycerol-3-phosphate-dehydrogenase, alpha enolase and L-lactate dehydrogenase B-chain) and in oxidative stress (superoxide dismutase, thioredoxins and selenium binding protein). Conclusions: A distinct proteomic profile of inflamed tissue in UC patients was found. Specific proteins involved in energy metabolism and oxidative stress were identified as potential candidate markers for UC.
Original languageEnglish
JournalB M C Gastroenterology
Publication date2012
Volume12
Issue76
Number of pages11
ISSN1471-230X
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 3
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