Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis. / Smith, Birgitte; Bodé, Susan; Petersen, Bodil L.; Jensen, Tim Kåre; Pipper, Christian; Kloppenborg, Julie; Boye, Mette; Krogfelt, Karen A.; Mølbak, Lars.

In: B M C Microbiology, Vol. 11, No. 1, 2011, p. 73.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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Smith, Birgitte; Bodé, Susan; Petersen, Bodil L.; Jensen, Tim Kåre; Pipper, Christian; Kloppenborg, Julie; Boye, Mette; Krogfelt, Karen A.; Mølbak, Lars / Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis.

In: B M C Microbiology, Vol. 11, No. 1, 2011, p. 73.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{ab07873807cc4ca0a52d0742937ca74f,
title = "Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis",
publisher = "BioMed Central Ltd.",
author = "Birgitte Smith and Susan Bodé and Petersen, {Bodil L.} and Jensen, {Tim Kåre} and Christian Pipper and Julie Kloppenborg and Mette Boye and Krogfelt, {Karen A.} and Lars Mølbak",
year = "2011",
doi = "10.1186/1471-2180-11-73",
volume = "11",
number = "1",
pages = "73",
journal = "B M C Microbiology",
issn = "1471-2180",

}

RIS

TY - JOUR

T1 - Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis

A1 - Smith,Birgitte

A1 - Bodé,Susan

A1 - Petersen,Bodil L.

A1 - Jensen,Tim Kåre

A1 - Pipper,Christian

A1 - Kloppenborg,Julie

A1 - Boye,Mette

A1 - Krogfelt,Karen A.

A1 - Mølbak,Lars

AU - Smith,Birgitte

AU - Bodé,Susan

AU - Petersen,Bodil L.

AU - Jensen,Tim Kåre

AU - Pipper,Christian

AU - Kloppenborg,Julie

AU - Boye,Mette

AU - Krogfelt,Karen A.

AU - Mølbak,Lars

PB - BioMed Central Ltd.

PY - 2011

Y1 - 2011

N2 - BACKGROUND: Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn neonates. Bacteria are believed to be important in the pathogenesis of NEC but bacterial characterization has only been done on human faecal samples and experimental animal studies. The aim of this study was to investigate the microbial composition and the relative number of bacteria in inflamed intestinal tissue surgically removed from neonates diagnosed with NEC (n = 24). The bacterial populations in the specimens were characterized by laser capture microdissection and subsequent sequencing combined with fluorescent in situ hybridization (FISH), using bacterial rRNA-targeting oligonucleotide probes. RESULTS: Bacteria were detected in 22 of the 24 specimens, 71% had moderate to high densities of bacteria. The phyla detected by 16S rRNA gene sequencing were: Proteobacteria (49.0%), Firmicutes (30.4%), Actinobacteria (17.1%) and Bacteroidetes (3.6%). A major detected class of the phylum Proteobacteria belonged to δ-proteobacteria. Surprisingly, Clostridium species were only detected in 4 of the specimens by FISH, but two of these specimens exhibited histological pneumatosis intestinalis and both specimens had a moderate to a high density of C. butyricum and C. parputrificum detected by using species specific FISH probes. A 16S rRNA gene sequence tag similar to Ralstonia species was detected in most of the neonatal tissues and members of this genus have been reported to be opportunistic pathogens but their role in NEC has still to be clarified. CONCLUSION: In this study, in situ identification and community analysis of bacteria found in tissue specimens from neonates with NEC, were analysed for the first time. Although a large variability of bacteria was found in most of the analyzed specimens, no single or combination of known potential pathogenic bacteria species was dominating the samples suggestive NEC as non-infectious syndrome. However there was a significant correlation between the presence of C. butyricum & C. parputrificum and histological pneumatosis intestinalis. Finally this study emphasizes the possibility to examine the microbial composition directly on excised human tissues to avoid biases from faecal samples or culturing.

AB - BACKGROUND: Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn neonates. Bacteria are believed to be important in the pathogenesis of NEC but bacterial characterization has only been done on human faecal samples and experimental animal studies. The aim of this study was to investigate the microbial composition and the relative number of bacteria in inflamed intestinal tissue surgically removed from neonates diagnosed with NEC (n = 24). The bacterial populations in the specimens were characterized by laser capture microdissection and subsequent sequencing combined with fluorescent in situ hybridization (FISH), using bacterial rRNA-targeting oligonucleotide probes. RESULTS: Bacteria were detected in 22 of the 24 specimens, 71% had moderate to high densities of bacteria. The phyla detected by 16S rRNA gene sequencing were: Proteobacteria (49.0%), Firmicutes (30.4%), Actinobacteria (17.1%) and Bacteroidetes (3.6%). A major detected class of the phylum Proteobacteria belonged to δ-proteobacteria. Surprisingly, Clostridium species were only detected in 4 of the specimens by FISH, but two of these specimens exhibited histological pneumatosis intestinalis and both specimens had a moderate to a high density of C. butyricum and C. parputrificum detected by using species specific FISH probes. A 16S rRNA gene sequence tag similar to Ralstonia species was detected in most of the neonatal tissues and members of this genus have been reported to be opportunistic pathogens but their role in NEC has still to be clarified. CONCLUSION: In this study, in situ identification and community analysis of bacteria found in tissue specimens from neonates with NEC, were analysed for the first time. Although a large variability of bacteria was found in most of the analyzed specimens, no single or combination of known potential pathogenic bacteria species was dominating the samples suggestive NEC as non-infectious syndrome. However there was a significant correlation between the presence of C. butyricum & C. parputrificum and histological pneumatosis intestinalis. Finally this study emphasizes the possibility to examine the microbial composition directly on excised human tissues to avoid biases from faecal samples or culturing.

U2 - 10.1186/1471-2180-11-73

DO - 10.1186/1471-2180-11-73

JO - B M C Microbiology

JF - B M C Microbiology

SN - 1471-2180

IS - 1

VL - 11

SP - 73

ER -