Co-digestion of manure and whey for in situ biogas upgrading by the addition of H2: process performance and microbial insights

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Co-digestion of manure and whey for in situ biogas upgrading by the addition of H2: process performance and microbial insights. / Luo, Gang; Angelidaki, Irini.

In: Applied Microbiology and Biotechnology, Vol. 97, No. 3, 2013, p. 1373-1381.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Luo, Gang; Angelidaki, Irini / Co-digestion of manure and whey for in situ biogas upgrading by the addition of H2: process performance and microbial insights.

In: Applied Microbiology and Biotechnology, Vol. 97, No. 3, 2013, p. 1373-1381.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{0ce75fec555548b78d289138e28bfb70,
title = "Co-digestion of manure and whey for in situ biogas upgrading by the addition of H2: process performance and microbial insights",
keywords = "Anaerobic digestion, Co-digestion, Hydrogen, In situ biogas upgrading",
publisher = "Springer",
author = "Gang Luo and Irini Angelidaki",
year = "2013",
doi = "10.1007/s00253-012-4547-5",
volume = "97",
number = "3",
pages = "1373--1381",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",

}

RIS

TY - JOUR

T1 - Co-digestion of manure and whey for in situ biogas upgrading by the addition of H<sub>2</sub>: process performance and microbial insights

A1 - Luo,Gang

A1 - Angelidaki,Irini

AU - Luo,Gang

AU - Angelidaki,Irini

PB - Springer

PY - 2013

Y1 - 2013

N2 - In situ biogas upgrading was conducted by introducing H2 directly to the anaerobic reactor. As H2 addition is associated with consumption of the CO2 in the biogas reactor, pH increased to higher than 8.0 when manure alone was used as substrate. By co-digestion of manure with acidic whey, the pH in the anaerobic reactor with the addition of hydrogen could be maintained below 8.0, which did not have inhibition to the anaerobic process. The H2 distribution systems (diffusers with different pore sizes) and liquid mixing intensities were demonstrated to affect the gas-liquid mass transfer of H2 and the biogas composition. The best biogas composition (75:6.6:18.4) was obtained at stirring speed 150 rpmand using ceramic diffuser, while the biogas in the control reactor consisted<br/>of CH4 and CO2 at a ratio of 55:45. The consumed hydrogen was almost completely converted to CH4, and there was no significant accumulation of VFA in the effluent. The study showed that addition of hydrogen had positive effect on the methanogenesis, but had no obvious effect on the acetogenesis. Both hydrogenotrophic methanogenic activity and the concentration of coenzyme F420 involved in methanogenesis were increased. The archaeal community was also altered with the addition of hydrogen, and a Methanothermobacter thermautotrophicus related band appeared in a denaturing gradient gel electrophoresis gel from the sample of the reactor with hydrogen addition. Though the addition of hydrogen increased the dissolved hydrogen concentration, the degradation of propionate was still thermodynamically feasible at the reactor conditions.

AB - In situ biogas upgrading was conducted by introducing H2 directly to the anaerobic reactor. As H2 addition is associated with consumption of the CO2 in the biogas reactor, pH increased to higher than 8.0 when manure alone was used as substrate. By co-digestion of manure with acidic whey, the pH in the anaerobic reactor with the addition of hydrogen could be maintained below 8.0, which did not have inhibition to the anaerobic process. The H2 distribution systems (diffusers with different pore sizes) and liquid mixing intensities were demonstrated to affect the gas-liquid mass transfer of H2 and the biogas composition. The best biogas composition (75:6.6:18.4) was obtained at stirring speed 150 rpmand using ceramic diffuser, while the biogas in the control reactor consisted<br/>of CH4 and CO2 at a ratio of 55:45. The consumed hydrogen was almost completely converted to CH4, and there was no significant accumulation of VFA in the effluent. The study showed that addition of hydrogen had positive effect on the methanogenesis, but had no obvious effect on the acetogenesis. Both hydrogenotrophic methanogenic activity and the concentration of coenzyme F420 involved in methanogenesis were increased. The archaeal community was also altered with the addition of hydrogen, and a Methanothermobacter thermautotrophicus related band appeared in a denaturing gradient gel electrophoresis gel from the sample of the reactor with hydrogen addition. Though the addition of hydrogen increased the dissolved hydrogen concentration, the degradation of propionate was still thermodynamically feasible at the reactor conditions.

KW - Anaerobic digestion

KW - Co-digestion

KW - Hydrogen

KW - In situ biogas upgrading

U2 - 10.1007/s00253-012-4547-5

DO - 10.1007/s00253-012-4547-5

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 3

VL - 97

SP - 1373

EP - 1381

ER -