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Characterization of the long-term immune response to vaccination against Mycobacterium avium subsp. paratuberculosis in Danish dairy cows. / Thomsen, Vibeke Thulstrup; Nielsen, Søren Saxmose; Thakur, Aneesh; Jungersen, Gregers.

In: Veterinary Immunology and Immunopathology, Vol. 145, No. 1-2, 2012, p. 316-322.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Thomsen, Vibeke Thulstrup; Nielsen, Søren Saxmose; Thakur, Aneesh; Jungersen, Gregers / Characterization of the long-term immune response to vaccination against Mycobacterium avium subsp. paratuberculosis in Danish dairy cows.

In: Veterinary Immunology and Immunopathology, Vol. 145, No. 1-2, 2012, p. 316-322.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{23be4f34a5f841759e051edbb4397b14,
title = "Characterization of the long-term immune response to vaccination against Mycobacterium avium subsp. paratuberculosis in Danish dairy cows",
publisher = "Elsevier BV",
author = "Thomsen, {Vibeke Thulstrup} and Nielsen, {Søren Saxmose} and Aneesh Thakur and Gregers Jungersen",
year = "2012",
doi = "10.1016/j.vetimm.2011.11.021",
volume = "145",
number = "1-2",
pages = "316--322",
journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",

}

RIS

TY - JOUR

T1 - Characterization of the long-term immune response to vaccination against Mycobacterium avium subsp. paratuberculosis in Danish dairy cows

A1 - Thomsen,Vibeke Thulstrup

A1 - Nielsen,Søren Saxmose

A1 - Thakur,Aneesh

A1 - Jungersen,Gregers

AU - Thomsen,Vibeke Thulstrup

AU - Nielsen,Søren Saxmose

AU - Thakur,Aneesh

AU - Jungersen,Gregers

PB - Elsevier BV

PY - 2012

Y1 - 2012

N2 - Vaccination of cattle against Mycobacterium avium subsp. paratuberculosis (MAP) provides partial protection by delayed shedding of MAP and reduced numbers of clinically affected animals. The duration of vaccine induced immune response is not known. The primary objective of this study was therefore to characterize the long-term effect of whole-cell based vaccination against MAP on the immune response. A secondary objective was to evaluate whether immunodiagnosis of MAP and Mycobacterium bovis infections is affected by MAP vaccination.Two studies were performed: (1) A retrospective longitudinal study including 895 vaccinated and 2526 non-vaccinated dairy cows in 9 Danish dairy herds aiming at characterizing the long-term antibody-response to vaccination; and (2) a cross-sectional study of responses in the IFN-γ assay carried out in 140 vaccinated animals in two herds to evaluate the effect of vaccination on the cell-mediated immune response and to evaluate a possible interference with the diagnosis of M. bovis infections.The results showed that 37% of samples from vaccinated animals and 5% of samples from non-vaccinated animals, respectively, were test positive in the milk antibody ELISA. The prevalence of antibody responses of the vaccinated animals was relatively constant from 2 to 6years of age, but decreased in older animals. Among the 140 vaccinated animals 88% tested positive with the IFN-γ test to johnin PPD and 50% responded to PPDb with IFN-γ production above a similar cut-off. Although Denmark is free of M. bovis, two of the vaccinated animals responded with higher IFN-γ levels when cultured with PPDb compared to PPDa. In conclusion, immunization with whole-cell MAP vaccines elicits both humoral and cell-mediated immune reactions, which may interfere with surveillance and diagnosis of both MAP and M. bovis infections using currently available tests.

AB - Vaccination of cattle against Mycobacterium avium subsp. paratuberculosis (MAP) provides partial protection by delayed shedding of MAP and reduced numbers of clinically affected animals. The duration of vaccine induced immune response is not known. The primary objective of this study was therefore to characterize the long-term effect of whole-cell based vaccination against MAP on the immune response. A secondary objective was to evaluate whether immunodiagnosis of MAP and Mycobacterium bovis infections is affected by MAP vaccination.Two studies were performed: (1) A retrospective longitudinal study including 895 vaccinated and 2526 non-vaccinated dairy cows in 9 Danish dairy herds aiming at characterizing the long-term antibody-response to vaccination; and (2) a cross-sectional study of responses in the IFN-γ assay carried out in 140 vaccinated animals in two herds to evaluate the effect of vaccination on the cell-mediated immune response and to evaluate a possible interference with the diagnosis of M. bovis infections.The results showed that 37% of samples from vaccinated animals and 5% of samples from non-vaccinated animals, respectively, were test positive in the milk antibody ELISA. The prevalence of antibody responses of the vaccinated animals was relatively constant from 2 to 6years of age, but decreased in older animals. Among the 140 vaccinated animals 88% tested positive with the IFN-γ test to johnin PPD and 50% responded to PPDb with IFN-γ production above a similar cut-off. Although Denmark is free of M. bovis, two of the vaccinated animals responded with higher IFN-γ levels when cultured with PPDb compared to PPDa. In conclusion, immunization with whole-cell MAP vaccines elicits both humoral and cell-mediated immune reactions, which may interfere with surveillance and diagnosis of both MAP and M. bovis infections using currently available tests.

KW - Paratuberculosis

KW - Long-term immune response

KW - Vaccination

U2 - 10.1016/j.vetimm.2011.11.021

DO - 10.1016/j.vetimm.2011.11.021

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 1-2

VL - 145

SP - 316

EP - 322

ER -