Characterization of the AN6448 cluster in Aspergillus nidulans

Publication: Research - peer-reviewConference abstract in journal – Annual report year: 2012

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With the aim of mapping the polyketome of A. nidulans we have made a library of strains, which individually overexpress PKS genes from an ectopic locus. A screen of this collection on different media demonstrated that AN6448 leads to production of 3-MOA. An inspection of the DNA sequence surrounding this gene uncovered a putative gene cluster including a gene, AN6446, with homology to transcription factors. Based on this observation, we decided to overexpress AN6446. A qRT-PCR analysis of this strain was used to delineate the borders of the gene cluster as well as to stimulate formation of cichorine and a number of new products, from the gene cluster. Subsequent deletion of all genes allowed several steps in the biosynthetic pathway in this cluster to be clarified.
Original languageEnglish
JournalPlanta Medica
Publication date2012
Volume78
Journal number11
Pages1113-1113
ISSN0032-0943
DOIs
StatePublished

Conference

ConferenceInternational Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF
CountryUnited States
CityNew York, NY
Period28/07/1201/08/12
CitationsWeb of Science® Times Cited: No match on DOI
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