Characterization of Isolates of Salmonella enterica Serovar Stanley, a Serovar Endemic to Asia and Associated with Travel

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Characterization of Isolates of Salmonella enterica Serovar Stanley, a Serovar Endemic to Asia and Associated with Travel. / Hendriksen, Rene S.; Le Hello, Simon; Bortolaia, Valeria; Pulsrikarn, Chaiwat; Nielsen, Eva Møller; Pornruangmong, Srirat; Chaichana, Phattharaporn; Svendsen, Christina Aaby; Weill, François-Xavier; Aarestrup, Frank Møller.

In: Journal of Clinical Microbiology, Vol. 50, No. 3, 2012, p. 709-720.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Hendriksen, Rene S.; Le Hello, Simon; Bortolaia, Valeria; Pulsrikarn, Chaiwat; Nielsen, Eva Møller; Pornruangmong, Srirat; Chaichana, Phattharaporn; Svendsen, Christina Aaby; Weill, François-Xavier; Aarestrup, Frank Møller / Characterization of Isolates of Salmonella enterica Serovar Stanley, a Serovar Endemic to Asia and Associated with Travel.

In: Journal of Clinical Microbiology, Vol. 50, No. 3, 2012, p. 709-720.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{9a56b1ba758d4d51a2fbb91a5e87ddd0,
title = "Characterization of Isolates of Salmonella enterica Serovar Stanley, a Serovar Endemic to Asia and Associated with Travel",
publisher = "American Society for Microbiology",
author = "Hendriksen, {Rene S.} and {Le Hello}, Simon and Valeria Bortolaia and Chaiwat Pulsrikarn and Nielsen, {Eva Møller} and Srirat Pornruangmong and Phattharaporn Chaichana and Svendsen, {Christina Aaby} and François-Xavier Weill and Aarestrup, {Frank Møller}",
year = "2012",
doi = "10.1128/JCM.05943-11",
volume = "50",
number = "3",
pages = "709--720",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",

}

RIS

TY - JOUR

T1 - Characterization of Isolates of Salmonella enterica Serovar Stanley, a Serovar Endemic to Asia and Associated with Travel

A1 - Hendriksen,Rene S.

A1 - Le Hello,Simon

A1 - Bortolaia,Valeria

A1 - Pulsrikarn,Chaiwat

A1 - Nielsen,Eva Møller

A1 - Pornruangmong,Srirat

A1 - Chaichana,Phattharaporn

A1 - Svendsen,Christina Aaby

A1 - Weill,François-Xavier

A1 - Aarestrup,Frank Møller

AU - Hendriksen,Rene S.

AU - Le Hello,Simon

AU - Bortolaia,Valeria

AU - Pulsrikarn,Chaiwat

AU - Nielsen,Eva Møller

AU - Pornruangmong,Srirat

AU - Chaichana,Phattharaporn

AU - Svendsen,Christina Aaby

AU - Weill,François-Xavier

AU - Aarestrup,Frank Møller

PB - American Society for Microbiology

PY - 2012

Y1 - 2012

N2 - Salmonella enterica serovar Stanley (S. Stanley) is a common serovar in Southeast Asia and was the second most common serovar implicated in human salmonellosis in Thailand in the years 2002 to 2007. In contrast, this serovar is relatively uncommon in Europe. The objective of this study was to characterize a collection of S. Stanley strains isolated from Thai (n = 62), Danish (n = 39), and French (n = 24) patients to gain a broader understanding of the genetic diversity, population dynamics, and susceptibility to antimicrobials. All isolates were characterized by pulsed-field gel electrophoresis and antimicrobial susceptibility testing. The molecular mechanisms of resistance to extended-spectrum cephalosporins and plasmid-mediated resistance to quinolones were characterized by PCR and sequencing. Plasmid profiling, replicon typing, and microarray analysis were used to characterize the genetic mechanisms of antimicrobial resistance in 10 extended-spectrum cephalosporinase-producing isolates. Considerable genetic diversity was observed among the isolates characterized with 91 unique XbaI pulsed-field gel electrophoresis (PFGE) patterns, including 17 distinct clusters consisting of two to seven indistinguishable isolates. We found some of the S. Stanley isolates isolated from patients in Europe were acquired during travel to Southeast Asia, including Thailand. The presence of multiple plasmid lineages carrying the extended-spectrum cephalosporinase-encoding blaCMY-2 gene in S. Stanley isolates from the central part of Thailand was confirmed. Our results emphasize that Thai authorities, as well as authorities in other countries lacking prudent use of antimicrobials, should improve the ongoing efforts to regulate antimicrobial use in agriculture and in clinical settings to limit the spread of multidrug-resistant Salmonella isolates and plasmids among humans and pigs in Thailand and abroad.

AB - Salmonella enterica serovar Stanley (S. Stanley) is a common serovar in Southeast Asia and was the second most common serovar implicated in human salmonellosis in Thailand in the years 2002 to 2007. In contrast, this serovar is relatively uncommon in Europe. The objective of this study was to characterize a collection of S. Stanley strains isolated from Thai (n = 62), Danish (n = 39), and French (n = 24) patients to gain a broader understanding of the genetic diversity, population dynamics, and susceptibility to antimicrobials. All isolates were characterized by pulsed-field gel electrophoresis and antimicrobial susceptibility testing. The molecular mechanisms of resistance to extended-spectrum cephalosporins and plasmid-mediated resistance to quinolones were characterized by PCR and sequencing. Plasmid profiling, replicon typing, and microarray analysis were used to characterize the genetic mechanisms of antimicrobial resistance in 10 extended-spectrum cephalosporinase-producing isolates. Considerable genetic diversity was observed among the isolates characterized with 91 unique XbaI pulsed-field gel electrophoresis (PFGE) patterns, including 17 distinct clusters consisting of two to seven indistinguishable isolates. We found some of the S. Stanley isolates isolated from patients in Europe were acquired during travel to Southeast Asia, including Thailand. The presence of multiple plasmid lineages carrying the extended-spectrum cephalosporinase-encoding blaCMY-2 gene in S. Stanley isolates from the central part of Thailand was confirmed. Our results emphasize that Thai authorities, as well as authorities in other countries lacking prudent use of antimicrobials, should improve the ongoing efforts to regulate antimicrobial use in agriculture and in clinical settings to limit the spread of multidrug-resistant Salmonella isolates and plasmids among humans and pigs in Thailand and abroad.

U2 - 10.1128/JCM.05943-11

DO - 10.1128/JCM.05943-11

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

VL - 50

SP - 709

EP - 720

ER -