Without internal affiliation

  • Author: Schröder, Olga

    Technische Universität Berlin, Germany

  • Author: Bleijlevens, Boris

    University of Amsterdam, Netherlands

  • Author: Jongh, Thyra E. de

    University of Amsterdam, Netherlands

  • Author: Chen, Zhujun

    Department of Chemistry, State University College at Buffalo, United States

  • Author: Li, Tianshu

    Department of Chemistry, State University College at Buffalo, United States

  • Author: Fischer, Jörg

    Lehrstuhl für Technische Mikrobiologie, Fachbereich Chemietechnik, Universität Dortmund, Germany

  • Author: Förster, Jochen

    Lehrstuhl für Technische Mikrobiologie, Fachbereich Chemietechnik, Universität Dortmund

  • Author: Friedrich, Cornelius G.

    Lehrstuhl für Technische Mikrobiologie, Fachbereich Chemietechnik, Universität Dortmund, Germany

  • Author: Bagley, Kimberly A.

    Department of Chemistry, State University College at Buffalo, United States

  • Author: Albracht, Simon P. J.

    University of Amsterdam, Netherlands

  • Author: Lubitz, Wolfgang

    Technische Universität Berlin, Germany

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Electron paramagnetic resonance (EPR) and Fourier transform IR studies on the soluble hydrogenase from Acidithiobacillus ferrooxidans are presented. In addition, detailed sequence analyses of the two subunits of the enzyme have been performed. They show that the enzyme belongs to a group of uptake [NiFe] hydrogenases typical for Cyanobacteria. The sequences have also a close relationship to those of the H2-sensor proteins, but clearly differ from those of standard [NiFe] hydrogenases. It is concluded that the structure of the catalytic centre is similar, but not identical, to that of known [NiFe] hydrogenases. The active site in the majority of oxidized enzyme molecules, 97% in cells and more than 50% in the purified enzyme, is EPR-silent. Upon contact with H2 these sites remain EPR-silent and show only a limited IR response. Oxidized enzyme molecules with an EPR-detectable active site show a Nir*-like EPR signal which is light-sensitive at cryogenic temperatures. This is a novelty in the field of [NiFe] hydrogenases. Reaction with H2 converts these active sites to the well-known Nia-C* state. Illumination below 160 K transforms this state into the Nia-L* state. The reversal, in the dark at 200 K, proceeds via an intermediate Ni EPR signal only observed with the H2-sensor protein from Ralstonia eutropha. The EPR-silent active sites in as-isolated and H2-treated enzyme are also light-sensitive as observed by IR spectra at cryogenic temperatures. The possible origin of the light sensitivity is discussed. This study represents the first spectral characterization of an enzyme of the group of cyanobacterial uptake hydrogenases.
Original languageEnglish
JournalJournal of Biological Inorganic Chemistry
Publication date2007
Volume12
Issue2
Pages212-233
ISSN0949-8257
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 17
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