Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing

Publication: Research - peer-reviewConference abstract in proceedings – Annual report year: 2012

Standard

Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing. / Ingerslev, Hans-Christian; Dalsgaard, Inger; Boye, Mette; Madsen, Lone.

DAFINET Workshop: Book of abstracts. Danish Fish Immunology Research Centre and Network, 2012.

Publication: Research - peer-reviewConference abstract in proceedings – Annual report year: 2012

Harvard

Ingerslev, H-C, Dalsgaard, I, Boye, M & Madsen, L 2012, 'Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing'. in DAFINET Workshop: Book of abstracts. Danish Fish Immunology Research Centre and Network.

APA

CBE

Ingerslev H-C, Dalsgaard I, Boye M, Madsen L. 2012. Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing. In DAFINET Workshop: Book of abstracts. Danish Fish Immunology Research Centre and Network.

MLA

Ingerslev, Hans-Christian et al. "Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing". DAFINET Workshop: Book of abstracts. Danish Fish Immunology Research Centre and Network. 2012.

Vancouver

Ingerslev H-C, Dalsgaard I, Boye M, Madsen L. Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing. In DAFINET Workshop: Book of abstracts. Danish Fish Immunology Research Centre and Network. 2012.

Author

Ingerslev, Hans-Christian; Dalsgaard, Inger; Boye, Mette; Madsen, Lone / Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing.

DAFINET Workshop: Book of abstracts. Danish Fish Immunology Research Centre and Network, 2012.

Publication: Research - peer-reviewConference abstract in proceedings – Annual report year: 2012

Bibtex

@inbook{12a8cbeb669f4e58a0629ac86e165a5e,
title = "Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing",
publisher = "Danish Fish Immunology Research Centre and Network",
author = "Hans-Christian Ingerslev and Inger Dalsgaard and Mette Boye and Lone Madsen",
year = "2012",
booktitle = "DAFINET Workshop",

}

RIS

TY - ABST

T1 - Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing

A1 - Ingerslev,Hans-Christian

A1 - Dalsgaard,Inger

A1 - Boye,Mette

A1 - Madsen,Lone

AU - Ingerslev,Hans-Christian

AU - Dalsgaard,Inger

AU - Boye,Mette

AU - Madsen,Lone

PB - Danish Fish Immunology Research Centre and Network

PY - 2012

Y1 - 2012

N2 - For many years it has been known that the bacterial microflora in the gut of warm-blooded animals exists in harmony with the host and exert various beneficial effects on the health by their metabolic activities. Hence, the gut microbiota has a high importance for the animal. In many studies from e.g. humans and the pig mapping of the bacterial flora from the gut have shown dominance by some specific bacterial groups, and this bacterial profile is termed as a ‘core microbiota’. For lower vertebrates like fish mapping of the bacterial flora in the gastrointestinal system is to date a relatively new research field and previous studies have mainly been done on bacterial species that can be cultured or by classical molecular techniques like T-RFLP and DGGE. In the last recent years deep-sequencing techniques have enabled sequencing and mapping of entire microbial communities from for instance an environmental sample or a tissue / fecal sample from an animal. These metagenomic studies have provided new and deeper insight into the microbial ecology and the influence of the microbiota in warm-blooded animals.<br/>In this study next-generation sequencing of the 16S rDNA gene on the Illumina HiSeq platform was used to examine the composition of the microbial flora in the gut of rainbow trout (Oncorhynchus mykiss) fry. The fish were examined before and after first-feeding and after administration by commercial probiotic lactobacilli to the feed.

AB - For many years it has been known that the bacterial microflora in the gut of warm-blooded animals exists in harmony with the host and exert various beneficial effects on the health by their metabolic activities. Hence, the gut microbiota has a high importance for the animal. In many studies from e.g. humans and the pig mapping of the bacterial flora from the gut have shown dominance by some specific bacterial groups, and this bacterial profile is termed as a ‘core microbiota’. For lower vertebrates like fish mapping of the bacterial flora in the gastrointestinal system is to date a relatively new research field and previous studies have mainly been done on bacterial species that can be cultured or by classical molecular techniques like T-RFLP and DGGE. In the last recent years deep-sequencing techniques have enabled sequencing and mapping of entire microbial communities from for instance an environmental sample or a tissue / fecal sample from an animal. These metagenomic studies have provided new and deeper insight into the microbial ecology and the influence of the microbiota in warm-blooded animals.<br/>In this study next-generation sequencing of the 16S rDNA gene on the Illumina HiSeq platform was used to examine the composition of the microbial flora in the gut of rainbow trout (Oncorhynchus mykiss) fry. The fish were examined before and after first-feeding and after administration by commercial probiotic lactobacilli to the feed.

UR - http://www.dafinet.dk/DAFINET/Abstract_books_files/DAFINET%20April%202012%20Abstracts.pdf

BT - DAFINET Workshop

T2 - DAFINET Workshop

ER -