Centrifugally driven microfluidic disc for detection of chromosomal translocations

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Standard

Centrifugally driven microfluidic disc for detection of chromosomal translocations. / Brøgger, Anna Line; Kwasny, Dorota; Bosco, Filippo G.; Silahtaroglu, Asli; Tümer, Zeynep; Boisen, Anja; Svendsen, Winnie E.

In: Lab On a Chip, Vol. 12, No. 22, 2012, p. 4628-4634.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Harvard

APA

CBE

MLA

Vancouver

Author

Brøgger, Anna Line; Kwasny, Dorota; Bosco, Filippo G.; Silahtaroglu, Asli; Tümer, Zeynep; Boisen, Anja; Svendsen, Winnie E. / Centrifugally driven microfluidic disc for detection of chromosomal translocations.

In: Lab On a Chip, Vol. 12, No. 22, 2012, p. 4628-4634.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{b2da4b42405247a189d1f8a193b6199d,
title = "Centrifugally driven microfluidic disc for detection of chromosomal translocations",
publisher = "Royal Society of Chemistry",
author = "Brøgger, {Anna Line} and Dorota Kwasny and Bosco, {Filippo G.} and Asli Silahtaroglu and Zeynep Tümer and Anja Boisen and Svendsen, {Winnie E.}",
year = "2012",
doi = "10.1039/c2lc40554g",
volume = "12",
number = "22",
pages = "4628--4634",
journal = "Lab On a Chip",
issn = "1473-0197",

}

RIS

TY - JOUR

T1 - Centrifugally driven microfluidic disc for detection of chromosomal translocations

A1 - Brøgger,Anna Line

A1 - Kwasny,Dorota

A1 - Bosco,Filippo G.

A1 - Silahtaroglu,Asli

A1 - Tümer,Zeynep

A1 - Boisen,Anja

A1 - Svendsen,Winnie E.

AU - Brøgger,Anna Line

AU - Kwasny,Dorota

AU - Bosco,Filippo G.

AU - Silahtaroglu,Asli

AU - Tümer,Zeynep

AU - Boisen,Anja

AU - Svendsen,Winnie E.

PB - Royal Society of Chemistry

PY - 2012

Y1 - 2012

N2 - Chromosome translocations are a common cause of congenital disorders and cancer. Current detection methods require use of expensive and highly specialized techniques to identify the chromosome regions involved in a translocation. There is a need for rapid yet specific detection for diagnosis and prognosis of patients. In this work we demonstrate a novel, centrifugally-driven microfluidic system for controlled manipulation of oligonucleotides and subsequent detection of chromosomal translocations. The device is fabricated in the form of a disc with capillary burst microvalves employed to control the fluid flow. The microvalves in series are designed to enable fluid movement from the center towards the periphery of the disc to handle DNA sequences representing translocation between chromosome 3 and 9. The translocation detection is performed in two hybridization steps in separate sorting and detection chambers. The burst frequencies of the two capillary burst microvalves are separated by 180 rpm enabling precise control of hybridization in each of the chambers. The DNA probes targeting a translocation are immobilized directly on PMMA by a UV-activated procedure, which is compatible with the disc fabrication method. The device performance was validated by successful specific hybridization of the translocation derivatives in the sorting and detection chambers.

AB - Chromosome translocations are a common cause of congenital disorders and cancer. Current detection methods require use of expensive and highly specialized techniques to identify the chromosome regions involved in a translocation. There is a need for rapid yet specific detection for diagnosis and prognosis of patients. In this work we demonstrate a novel, centrifugally-driven microfluidic system for controlled manipulation of oligonucleotides and subsequent detection of chromosomal translocations. The device is fabricated in the form of a disc with capillary burst microvalves employed to control the fluid flow. The microvalves in series are designed to enable fluid movement from the center towards the periphery of the disc to handle DNA sequences representing translocation between chromosome 3 and 9. The translocation detection is performed in two hybridization steps in separate sorting and detection chambers. The burst frequencies of the two capillary burst microvalves are separated by 180 rpm enabling precise control of hybridization in each of the chambers. The DNA probes targeting a translocation are immobilized directly on PMMA by a UV-activated procedure, which is compatible with the disc fabrication method. The device performance was validated by successful specific hybridization of the translocation derivatives in the sorting and detection chambers.

U2 - 10.1039/c2lc40554g

DO - 10.1039/c2lc40554g

JO - Lab On a Chip

JF - Lab On a Chip

SN - 1473-0197

IS - 22

VL - 12

SP - 4628

EP - 4634

ER -