Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus

Publication: Research - peer-reviewJournal article – Annual report year: 2010

  • Author: Scapigliati, G.

    Dipartimento di Scienze Ambientali, Largo dell'Università

  • Author: Buonocore, F.

    Dipartimento di Scienze Ambientali, Largo dell'Università

  • Author: Randelli, E.

    Dipartimento di Scienze Ambientali, Largo dell'Università

  • Author: Casani, D.

    Dipartimento di Scienze Ambientali, Largo dell'Università

  • Author: Meloni, S.

    Dipartimento di Scienze Ambientali, Largo dell'Università

  • Author: Zarletti, G.

    Dipartimento di Scienze Ambientali, Largo dell'Università

  • Author: Tiberi, M.

    Dipartimento di Scienze Ambientali, Largo dell'Università

  • Author: Pietretti, D.

    Dipartimento di Scienze Ambientali, Largo dell'Università

  • Author: Boschi, I.

    Dipartimento di Scienze Ambientali, Largo dell'Università

  • Author: Manchado, M.

    Centro IFAPA, Junta de Andalucia, El Torufio, Càdiz, Spain

  • Author: Martin-Antonio, B.

    Centro IFAPA, Junta de Andalucia, El Torufio, Càdiz, Spain

  • Author: Jimenez-Cantizano, R.

    Centro IFAPA, Junta de Andalucia, El Torufio, Càdiz, Spain

  • Author: Bovo, G.

    Istituto Zooprofilattico Spermentale delle Venezie, Italy

  • Author: Borghesan, F.

    Istituto Zooprofilattico Spermentale delle Venezie, Italy

  • Author: Lorenzen, Niels

    Section of Fish Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark

  • Author: Einer-Jensen, Katja

    Section of Fish Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark

  • Author: Adams, S.

    Aquatic Diagnostics, UK

  • Author: Thompson, K.

    Aquatic Diagnostics, UK

  • Author: Alonso, C.

    University of Malaga, Spain

  • Author: Bejar, J.

    University of Malaga, Spain

  • Author: Borrego, J.J.

    University of Malaga, Spain

  • Author: Alvarez, M.C.

    University of Malaga, Spain

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Naïve sea bass juveniles (38.4 ± 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-β, TCRβ, CD4, CD8α, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable “in vitro” increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for type I IFN, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF-β and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.
Original languageEnglish
JournalFish and Shellfish Immunology
Publication date2010
Issue28
Pages303-311
ISSN1050-4648
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 18
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