Cell motility, morphology, viability and proliferation in response to nanotopography on silicon black

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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@article{6366e52b2d7f4a0e843a9d874336fe9e,
title = "Cell motility, morphology, viability and proliferation in response to nanotopography on silicon black",
publisher = "royal society of chemistry",
author = "Lopacinska, {Joanna M.} and Cristian Gradinaru and Rafal Wierzbicki and Carsten Købler and Schmidt, {Michael S.} and Madsen, {Martin T.} and Maciej Skolimowski and Martin Dufva and Henrik Flyvbjerg and Kristian Mølhave",
year = "2012",
doi = "10.1039/c2nr11455k",
volume = "4",
number = "12",
pages = "3739--3745",
journal = "Nanoscale",
issn = "2040-3364",

}

RIS

TY - JOUR

T1 - Cell motility, morphology, viability and proliferation in response to nanotopography on silicon black

A1 - Lopacinska,Joanna M.

A1 - Gradinaru,Cristian

A1 - Wierzbicki,Rafal

A1 - Købler,Carsten

A1 - Schmidt,Michael S.

A1 - Madsen,Martin T.

A1 - Skolimowski,Maciej

A1 - Dufva,Martin

A1 - Flyvbjerg,Henrik

A1 - Mølhave,Kristian

AU - Lopacinska,Joanna M.

AU - Gradinaru,Cristian

AU - Wierzbicki,Rafal

AU - Købler,Carsten

AU - Schmidt,Michael S.

AU - Madsen,Martin T.

AU - Skolimowski,Maciej

AU - Dufva,Martin

AU - Flyvbjerg,Henrik

AU - Mølhave,Kristian

PB - royal society of chemistry

PY - 2012

Y1 - 2012

N2 - Knowledge of cells' interactions with nanostructured materials is fundamental for bio-nanotechnology. We present results for how individual mouse fibroblasts from cell line NIH3T3 respond to highly spiked surfaces of silicon black that were fabricated by maskless reactive ion etching (RIE). We did standard measurements of cell viability, proliferation, and morphology on various surfaces. We also analyzed the motility of cells on the same surfaces, as recorded in time lapse movies of sparsely populated cell cultures. We find that motility and morphology vary strongly with nano-patterns, while viability and proliferation show little dependence on substrate type. We conclude that motility analysis can show a wide range of cell responses e. g. over a factor of two in cell speed to different nano-topographies, where standard assays, such as viability or proliferation, in the tested cases show much less variation of the order 10-20%.

AB - Knowledge of cells' interactions with nanostructured materials is fundamental for bio-nanotechnology. We present results for how individual mouse fibroblasts from cell line NIH3T3 respond to highly spiked surfaces of silicon black that were fabricated by maskless reactive ion etching (RIE). We did standard measurements of cell viability, proliferation, and morphology on various surfaces. We also analyzed the motility of cells on the same surfaces, as recorded in time lapse movies of sparsely populated cell cultures. We find that motility and morphology vary strongly with nano-patterns, while viability and proliferation show little dependence on substrate type. We conclude that motility analysis can show a wide range of cell responses e. g. over a factor of two in cell speed to different nano-topographies, where standard assays, such as viability or proliferation, in the tested cases show much less variation of the order 10-20%.

U2 - 10.1039/c2nr11455k

DO - 10.1039/c2nr11455k

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 12

VL - 4

SP - 3739

EP - 3745

ER -