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Bibtex

@article{f0bdd6e81f5047a5a4cdc418f57ef827,
title = "Catalyst-Free Conjugation and In Situ Quantification of Nanoparticle Ligand Surface Density Using Fluorogenic Cu-Free Click Chemistry",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
author = "Jølck, {Rasmus Irming} and Honghao Sun and Berg, {Rolf Henrik} and Andresen, {Thomas Lars}",
year = "2011",
doi = "10.1002/chem.201003131",
volume = "17",
number = "12",
pages = "3326--3331",
journal = "Chemistry - A European journal",
issn = "0947-6539",

}

RIS

TY - JOUR

T1 - Catalyst-Free Conjugation and In Situ Quantification of Nanoparticle Ligand Surface Density Using Fluorogenic Cu-Free Click Chemistry

A1 - Jølck,Rasmus Irming

A1 - Sun,Honghao

A1 - Berg,Rolf Henrik

A1 - Andresen,Thomas Lars

AU - Jølck,Rasmus Irming

AU - Sun,Honghao

AU - Berg,Rolf Henrik

AU - Andresen,Thomas Lars

PB - Wiley - V C H Verlag GmbH & Co. KGaA

PY - 2011

Y1 - 2011

N2 - A highly efficient method for functionalizing nanoparticles and directly quantifying conjugation efficiency and ligand surface density has been developed. Attachment of 3-azido-modifed RGD-peptides to PEGylated liposomes was achieved by using Cu-free click conditions. Upon coupling a fluorophore is formed, which could be utilized to monitor conjugation efficiency and the obtained ligand surface density in situ, without prior purification.

AB - A highly efficient method for functionalizing nanoparticles and directly quantifying conjugation efficiency and ligand surface density has been developed. Attachment of 3-azido-modifed RGD-peptides to PEGylated liposomes was achieved by using Cu-free click conditions. Upon coupling a fluorophore is formed, which could be utilized to monitor conjugation efficiency and the obtained ligand surface density in situ, without prior purification.

KW - Coumarin

KW - Surface functionalization

KW - Nanoparticles

KW - Lposomes

KW - Click chemistry

U2 - 10.1002/chem.201003131

DO - 10.1002/chem.201003131

JO - Chemistry - A European journal

JF - Chemistry - A European journal

SN - 0947-6539

IS - 12

VL - 17

SP - 3326

EP - 3331

ER -