Bovine digital dermatitis: Possible pathogenic consortium consisting of Dichelobacter nodosus and multiple Treponema species

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Bovine digital dermatitis: Possible pathogenic consortium consisting of Dichelobacter nodosus and multiple Treponema species. / Rasmussen, Marianne; Capion, Nynne; Schou, Kirstine Klitgaard; Rogdo, Torunn ; Fjeldaas, Terje ; Boye, Mette; Jensen, Tim Kåre.

In: Veterinary Microbiology, Vol. 160, No. 1-2, 2012, p. 151–161.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Rasmussen, Marianne; Capion, Nynne; Schou, Kirstine Klitgaard; Rogdo, Torunn ; Fjeldaas, Terje ; Boye, Mette; Jensen, Tim Kåre / Bovine digital dermatitis: Possible pathogenic consortium consisting of Dichelobacter nodosus and multiple Treponema species.

In: Veterinary Microbiology, Vol. 160, No. 1-2, 2012, p. 151–161.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{b71b412de9814205b7566abae3bbfef0,
title = "Bovine digital dermatitis: Possible pathogenic consortium consisting of Dichelobacter nodosus and multiple Treponema species",
publisher = "Elsevier BV",
author = "Marianne Rasmussen and Nynne Capion and Schou, {Kirstine Klitgaard} and Torunn Rogdo and Terje Fjeldaas and Mette Boye and Jensen, {Tim Kåre}",
year = "2012",
doi = "10.1016/j.vetmic.2012.05.018",
volume = "160",
number = "1-2",
pages = "151–161",
journal = "Veterinary Microbiology",
issn = "0378-1135",

}

RIS

TY - JOUR

T1 - Bovine digital dermatitis: Possible pathogenic consortium consisting of Dichelobacter nodosus and multiple Treponema species

A1 - Rasmussen,Marianne

A1 - Capion,Nynne

A1 - Schou,Kirstine Klitgaard

A1 - Rogdo,Torunn

A1 - Fjeldaas,Terje

A1 - Boye,Mette

A1 - Jensen,Tim Kåre

AU - Rasmussen,Marianne

AU - Capion,Nynne

AU - Schou,Kirstine Klitgaard

AU - Rogdo,Torunn

AU - Fjeldaas,Terje

AU - Boye,Mette

AU - Jensen,Tim Kåre

PB - Elsevier BV

PY - 2012

Y1 - 2012

N2 - Bovine digital dermatitis (DD) is a multifactorial disease involving at least one or more treponemal species. Virulent phylotypes of Treponema and other infectious agents contributing to disease etiology still remain to be identified. This study addressed these questions by analyzing the prevalence and distribution of seventeen phylotypes of Treponema in DD lesions by fluorescent in situ hybridization (FISH) applying species/phylotype-specific oligonucleotide probes. In situ hybridization for Dichelobacter nodosus, the cause of ovine footrot, was additionally performed. We sampled 90 biopsies of DD lesions originating from one Norwegian and six Danish dairy herds, and 24 tissue samples of healthy skin. All lesions revealed intermingled infections with multiple Treponema phylotypes (mean > 7). In six herds, the mean number of phylotypes identified varied between 12 and 15. D. nodosus was present in forty-nine (51%) of the lesions and in three of the apparently healthy skin samples. Two “healthy” samples also contained Treponema spp. and D. nodosus, and were histopathologically categorized as subclinical DD. Another eighteen of the “healthy” skin samples showed serious epidermal hyperplasia but were not colonized by bacteria while only four samples were found normal. We hypothesise that external noxious stimuli allow D. nodosus to break down the epidermal barrier creating a suitable environment for the secondary invaders, Treponema species, which gradually take over the infection site. The variety and different distribution of treponemes in the DD lesions observed in this study, suggests that most of the Treponema phylotypes have the potential to be pathogenic.

AB - Bovine digital dermatitis (DD) is a multifactorial disease involving at least one or more treponemal species. Virulent phylotypes of Treponema and other infectious agents contributing to disease etiology still remain to be identified. This study addressed these questions by analyzing the prevalence and distribution of seventeen phylotypes of Treponema in DD lesions by fluorescent in situ hybridization (FISH) applying species/phylotype-specific oligonucleotide probes. In situ hybridization for Dichelobacter nodosus, the cause of ovine footrot, was additionally performed. We sampled 90 biopsies of DD lesions originating from one Norwegian and six Danish dairy herds, and 24 tissue samples of healthy skin. All lesions revealed intermingled infections with multiple Treponema phylotypes (mean > 7). In six herds, the mean number of phylotypes identified varied between 12 and 15. D. nodosus was present in forty-nine (51%) of the lesions and in three of the apparently healthy skin samples. Two “healthy” samples also contained Treponema spp. and D. nodosus, and were histopathologically categorized as subclinical DD. Another eighteen of the “healthy” skin samples showed serious epidermal hyperplasia but were not colonized by bacteria while only four samples were found normal. We hypothesise that external noxious stimuli allow D. nodosus to break down the epidermal barrier creating a suitable environment for the secondary invaders, Treponema species, which gradually take over the infection site. The variety and different distribution of treponemes in the DD lesions observed in this study, suggests that most of the Treponema phylotypes have the potential to be pathogenic.

KW - Bovine

KW - Digital dermatitis

KW - Treponema

KW - Dichelobacter nodosus

U2 - 10.1016/j.vetmic.2012.05.018

DO - 10.1016/j.vetmic.2012.05.018

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-2

VL - 160

SP - 151

EP - 161

ER -