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A blocking enzyme-linked immunosorbent assay (ELISA) detecting antibodies against Actinobacillus pleuropneumoniae (Ap) serotype 6 was developed. The blocking ELISA was based on the inhibition of a polyclonal antibody raised against Ap serotype 6. Purified lipopolysaccharide from Ap serotype 6 was used as antigen. The blocking ELISA was tested against sera from pigs experimentally infected with the 12 serotypes of Ap biotype 1. Cross-reaction with serotypes 3 and 8 but not with other serotypes was observed. The sensitivity and specificity of the test on a herd level were evaluated with sera from herds naturally infected with serotypes 2, 6, 8 or 12 and with sera from herds free of infection with any Ap serotype. The blocking ELISA showed a high herd sensitivity (1.00 (0.79-1.00)) and specificity (0.97 (0.93-0.99)).
Original languageEnglish
JournalVeterinary Microbiology
Publication date2001
Volume79
Issue1
Pages11-18
ISSN0378-1135
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 9

Keywords

  • serological assay, Actinobacillus pleuropneumoniae, pig-bacteria, ELISA, lipopolysaccharide, pleuropneumonia
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