Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Standard

Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures. / De Abreu, Angela Alexandra Valente; Karakashev, Dimitar Borisov; Angelidaki, Irini; Sousa, Diana Z.; Alves, M. Madalena.

In: Biotechnology for Biofuels, Vol. 5, No. 6, 2012.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Harvard

APA

CBE

MLA

Vancouver

Author

De Abreu, Angela Alexandra Valente; Karakashev, Dimitar Borisov; Angelidaki, Irini; Sousa, Diana Z.; Alves, M. Madalena / Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures.

In: Biotechnology for Biofuels, Vol. 5, No. 6, 2012.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{f18d03db62e34ebe9dd26c47db4dd2d9,
title = "Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures",
publisher = "BioMed Central Ltd.",
author = "{De Abreu}, {Angela Alexandra Valente} and Karakashev, {Dimitar Borisov} and Irini Angelidaki and Sousa, {Diana Z.} and Alves, {M. Madalena}",
year = "2012",
doi = "10.1186/1754-6834-5-6",
volume = "5",
number = "6",
journal = "Biotechnology for Biofuels",
issn = "1754-6834",

}

RIS

TY - JOUR

T1 - Biohydrogen production from arabinose and glucose using extreme thermophilic anaerobic mixed cultures

A1 - De Abreu,Angela Alexandra Valente

A1 - Karakashev,Dimitar Borisov

A1 - Angelidaki,Irini

A1 - Sousa,Diana Z.

A1 - Alves,M. Madalena

AU - De Abreu,Angela Alexandra Valente

AU - Karakashev,Dimitar Borisov

AU - Angelidaki,Irini

AU - Sousa,Diana Z.

AU - Alves,M. Madalena

PB - BioMed Central Ltd.

PY - 2012

Y1 - 2012

N2 - Background Second generation hydrogen fermentation technologies using organic agricultural and forestry wastes are emerging. The efficient microbial fermentation of hexoses and pentoses resulting from the pretreatment of lingocellulosic materials is essential for the success of these processes. Results Conversion of arabinose and glucose to hydrogen, by extreme thermophilic anaerobic mixed cultures was studied in continuous (70oC, pH 5.5) and batch (70oC, pH 5.5 and pH 7) assays. Two EGSB reactors, Rarab and Rgluc, were continuously fed with arabinose and glucose, respectively. No significant differences in reactor performance were observed for arabinose and glucose organic loading rates (OLR) ranging from 4.3 to 7.1 kgCOD m-3 d-1. However, for an OLR of 14.2 kgCOD m-3 d-1, hydrogen production rate and hydrogen yield were higher in Rarab than in Rgluc (average hydrogen production rate of 3.2 and 2.0 LH2 L-1 d-1 and hydrogen yield of 1.10 and 0.75 molH2 mol-1substrate for Rarab and Rgluc, respectively). Lower hydrogen production in Rgluc was associated with higher lactate production. DGGE results revealed no significant difference on the bacterial community composition between operational periods and between the reactors. Increased hydrogen production was observed in batch experiments when hydrogen partial pressure was kept low, both with arabinose and glucose as substrate. Sugars were completely consumed and hydrogen production stimulated (62% higher) when pH 7 was used instead of pH 5.5. Conclusions Continuous hydrogen production rate from arabinose was significantly higher than from glucose, when higher organic loading rate was used. The effect of hydrogen partial pressure on hydrogen production from glucose in batch mode was related to the extent of sugar utilization and not to the efficiency of substrate conversion to hydrogen. Furthermore, at pH 7.0, sugars uptake, hydrogen production and yield were higher than at pH 5.5, with both arabinose and glucose as substrates.

AB - Background Second generation hydrogen fermentation technologies using organic agricultural and forestry wastes are emerging. The efficient microbial fermentation of hexoses and pentoses resulting from the pretreatment of lingocellulosic materials is essential for the success of these processes. Results Conversion of arabinose and glucose to hydrogen, by extreme thermophilic anaerobic mixed cultures was studied in continuous (70oC, pH 5.5) and batch (70oC, pH 5.5 and pH 7) assays. Two EGSB reactors, Rarab and Rgluc, were continuously fed with arabinose and glucose, respectively. No significant differences in reactor performance were observed for arabinose and glucose organic loading rates (OLR) ranging from 4.3 to 7.1 kgCOD m-3 d-1. However, for an OLR of 14.2 kgCOD m-3 d-1, hydrogen production rate and hydrogen yield were higher in Rarab than in Rgluc (average hydrogen production rate of 3.2 and 2.0 LH2 L-1 d-1 and hydrogen yield of 1.10 and 0.75 molH2 mol-1substrate for Rarab and Rgluc, respectively). Lower hydrogen production in Rgluc was associated with higher lactate production. DGGE results revealed no significant difference on the bacterial community composition between operational periods and between the reactors. Increased hydrogen production was observed in batch experiments when hydrogen partial pressure was kept low, both with arabinose and glucose as substrate. Sugars were completely consumed and hydrogen production stimulated (62% higher) when pH 7 was used instead of pH 5.5. Conclusions Continuous hydrogen production rate from arabinose was significantly higher than from glucose, when higher organic loading rate was used. The effect of hydrogen partial pressure on hydrogen production from glucose in batch mode was related to the extent of sugar utilization and not to the efficiency of substrate conversion to hydrogen. Furthermore, at pH 7.0, sugars uptake, hydrogen production and yield were higher than at pH 5.5, with both arabinose and glucose as substrates.

UR - http://www.biotechnologyforbiofuels.com/content/5/1/6/abstract

U2 - 10.1186/1754-6834-5-6

DO - 10.1186/1754-6834-5-6

JO - Biotechnology for Biofuels

JF - Biotechnology for Biofuels

SN - 1754-6834

IS - 6

VL - 5

ER -