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Assessment of real-time myocardial uptake and enzymatic conversion of hyperpolarized [1-¹³C]pyruvate in pigs using slice selective magnetic resonance spectroscopy.. / Menichetti, Luca; Frijia, Francesca; Flori, Alessandra; Wiesinger, Florian; Lionetti, Vincenzo; Giovannetti, Giulio; Aquaro, Giovanni Donato; Recchia, Fabio A; Ardenkjaer-Larsen, Jan Henrik; Santarelli, Maria Filomena; Lombardi, Massimo.

In: Contrast Media & Molecular Imaging, Vol. 7, No. 1, 2012, p. 85-94.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

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Menichetti, Luca; Frijia, Francesca; Flori, Alessandra; Wiesinger, Florian; Lionetti, Vincenzo; Giovannetti, Giulio; Aquaro, Giovanni Donato; Recchia, Fabio A; Ardenkjaer-Larsen, Jan Henrik; Santarelli, Maria Filomena; Lombardi, Massimo / Assessment of real-time myocardial uptake and enzymatic conversion of hyperpolarized [1-¹³C]pyruvate in pigs using slice selective magnetic resonance spectroscopy..

In: Contrast Media & Molecular Imaging, Vol. 7, No. 1, 2012, p. 85-94.

Publication: Research - peer-reviewJournal article – Annual report year: 2012

Bibtex

@article{4573c10b3dd94f3a8b6381a988244ac3,
title = "Assessment of real-time myocardial uptake and enzymatic conversion of hyperpolarized [1-¹³C]pyruvate in pigs using slice selective magnetic resonance spectroscopy.",
keywords = "PROGNOSTIC VALUE, WALL-MOTION, C-13, Pyruvate, Metabolism, HEART, DEHYDROGENASE, Validation, Perfusion, Kinetics",
publisher = "JohnWiley & Sons Ltd.",
author = "Luca Menichetti and Francesca Frijia and Alessandra Flori and Florian Wiesinger and Vincenzo Lionetti and Giulio Giovannetti and Aquaro, {Giovanni Donato} and Recchia, {Fabio A} and Ardenkjaer-Larsen, {Jan Henrik} and Santarelli, {Maria Filomena} and Massimo Lombardi",
year = "2012",
doi = "10.1002/cmmi.480",
volume = "7",
number = "1",
pages = "85--94",
journal = "Contrast Media & Molecular Imaging",
issn = "1555-4309",

}

RIS

TY - JOUR

T1 - Assessment of real-time myocardial uptake and enzymatic conversion of hyperpolarized [1-¹³C]pyruvate in pigs using slice selective magnetic resonance spectroscopy.

A1 - Menichetti,Luca

A1 - Frijia,Francesca

A1 - Flori,Alessandra

A1 - Wiesinger,Florian

A1 - Lionetti,Vincenzo

A1 - Giovannetti,Giulio

A1 - Aquaro,Giovanni Donato

A1 - Recchia,Fabio A

A1 - Ardenkjaer-Larsen,Jan Henrik

A1 - Santarelli,Maria Filomena

A1 - Lombardi,Massimo

AU - Menichetti,Luca

AU - Frijia,Francesca

AU - Flori,Alessandra

AU - Wiesinger,Florian

AU - Lionetti,Vincenzo

AU - Giovannetti,Giulio

AU - Aquaro,Giovanni Donato

AU - Recchia,Fabio A

AU - Ardenkjaer-Larsen,Jan Henrik

AU - Santarelli,Maria Filomena

AU - Lombardi,Massimo

PB - JohnWiley & Sons Ltd.

PY - 2012

Y1 - 2012

N2 - Hyperpolarization of ¹³C-labeled energy substrates enables the noninvasive detection and mapping of metabolic activity, in vivo, with magnetic resonance spectroscopy (MRS). Therefore, hyperpolarization and ¹³C MRS can potentially become a powerful tool to study the physiology of organs such as the heart, through the quantification of kinetic patterns under both normal and pathological conditions. In this study we assessed myocardial uptake and metabolism of hyperpolarized [1-¹³C]pyruvate in anesthetized pigs. Pyruvate metabolism was studied at baseline and during dobutamine-induced stimulation. We applied a numerical approach for spectral analysis and kinetic fitting (LSFIT/KIMOfit), making a comparison with a well-known jMRUI/AMARES analysis and γ-variate function, and we estimated the apparent conversion rate of hyperpolarized [1-¹³C]pyruvate into its downstream metabolites [1-¹³C]lactate, [1-¹³C]alanine and [¹³C]bicarbonate in a 3 T MR scanner. We detected an increase in the apparent kinetic constants (k(PX) ) for bicarbonate and lactate of two-fold during dobutamine infusion. These data correlate with the double product (rate-pressure product), an indirect parameter of cardiac oxygen consumption: we observed an increase in value by 46 ± 11% during inotropic stress. The proposed approach might be applied to future studies in models of cardiac disease and/or for the assessment of the pharmacokinetic properties of suitable ¹³C-enriched tracers for MRS.

AB - Hyperpolarization of ¹³C-labeled energy substrates enables the noninvasive detection and mapping of metabolic activity, in vivo, with magnetic resonance spectroscopy (MRS). Therefore, hyperpolarization and ¹³C MRS can potentially become a powerful tool to study the physiology of organs such as the heart, through the quantification of kinetic patterns under both normal and pathological conditions. In this study we assessed myocardial uptake and metabolism of hyperpolarized [1-¹³C]pyruvate in anesthetized pigs. Pyruvate metabolism was studied at baseline and during dobutamine-induced stimulation. We applied a numerical approach for spectral analysis and kinetic fitting (LSFIT/KIMOfit), making a comparison with a well-known jMRUI/AMARES analysis and γ-variate function, and we estimated the apparent conversion rate of hyperpolarized [1-¹³C]pyruvate into its downstream metabolites [1-¹³C]lactate, [1-¹³C]alanine and [¹³C]bicarbonate in a 3 T MR scanner. We detected an increase in the apparent kinetic constants (k(PX) ) for bicarbonate and lactate of two-fold during dobutamine infusion. These data correlate with the double product (rate-pressure product), an indirect parameter of cardiac oxygen consumption: we observed an increase in value by 46 ± 11% during inotropic stress. The proposed approach might be applied to future studies in models of cardiac disease and/or for the assessment of the pharmacokinetic properties of suitable ¹³C-enriched tracers for MRS.

KW - PROGNOSTIC VALUE

KW - WALL-MOTION

KW - C-13

KW - Pyruvate

KW - Metabolism

KW - HEART

KW - DEHYDROGENASE

KW - Validation

KW - Perfusion

KW - Kinetics

U2 - 10.1002/cmmi.480

DO - 10.1002/cmmi.480

JO - Contrast Media & Molecular Imaging

JF - Contrast Media & Molecular Imaging

SN - 1555-4309

IS - 1

VL - 7

SP - 85

EP - 94

ER -