Aspergillus triggers phenazine production in Pseudomonas aeruginosa

Publication: Research - peer-reviewConference abstract for conference – Annual report year: 2011

Standard

Aspergillus triggers phenazine production in Pseudomonas aeruginosa. / Jensen, Britt Guillaume; Jelsbak, Lars; Søndergaard, Ib; Pedersen, Mona Højgaard; Frisvad, Jens Christian; Nielsen, Kristian Fog.

2011. Abstract from European Congress on Microbial Biofilms – Basic and Clinical Aspects, Copenhagen, Denmark, .

Publication: Research - peer-reviewConference abstract for conference – Annual report year: 2011

Harvard

Jensen, BG, Jelsbak, L, Søndergaard, I, Pedersen, MH, Frisvad, JC & Nielsen, KF 2011, 'Aspergillus triggers phenazine production in Pseudomonas aeruginosa' European Congress on Microbial Biofilms – Basic and Clinical Aspects, Copenhagen, Denmark, 01/01/11,

APA

Jensen, B. G., Jelsbak, L., Søndergaard, I., Pedersen, M. H., Frisvad, J. C., & Nielsen, K. F. (2011). Aspergillus triggers phenazine production in Pseudomonas aeruginosa. Abstract from European Congress on Microbial Biofilms – Basic and Clinical Aspects, Copenhagen, Denmark, .

CBE

Jensen BG, Jelsbak L, Søndergaard I, Pedersen MH, Frisvad JC, Nielsen KF. 2011. Aspergillus triggers phenazine production in Pseudomonas aeruginosa. Abstract from European Congress on Microbial Biofilms – Basic and Clinical Aspects, Copenhagen, Denmark, .

MLA

Vancouver

Jensen BG, Jelsbak L, Søndergaard I, Pedersen MH, Frisvad JC, Nielsen KF. Aspergillus triggers phenazine production in Pseudomonas aeruginosa. 2011. Abstract from European Congress on Microbial Biofilms – Basic and Clinical Aspects, Copenhagen, Denmark, .

Author

Jensen, Britt Guillaume; Jelsbak, Lars; Søndergaard, Ib; Pedersen, Mona Højgaard; Frisvad, Jens Christian; Nielsen, Kristian Fog / Aspergillus triggers phenazine production in Pseudomonas aeruginosa.

2011. Abstract from European Congress on Microbial Biofilms – Basic and Clinical Aspects, Copenhagen, Denmark, .

Publication: Research - peer-reviewConference abstract for conference – Annual report year: 2011

Bibtex

@misc{6719d819543f4c65a6297504556714d4,
title = "Aspergillus triggers phenazine production in Pseudomonas aeruginosa",
author = "Jensen, {Britt Guillaume} and Lars Jelsbak and Ib Søndergaard and Pedersen, {Mona Højgaard} and Frisvad, {Jens Christian} and Nielsen, {Kristian Fog}",
year = "2011",
type = "ConferencePaper <importModel: ConferenceImportModel>",

}

RIS

TY - ABST

T1 - Aspergillus triggers phenazine production in Pseudomonas aeruginosa

A1 - Jensen,Britt Guillaume

A1 - Jelsbak,Lars

A1 - Søndergaard,Ib

A1 - Pedersen,Mona Højgaard

A1 - Frisvad,Jens Christian

A1 - Nielsen,Kristian Fog

AU - Jensen,Britt Guillaume

AU - Jelsbak,Lars

AU - Søndergaard,Ib

AU - Pedersen,Mona Højgaard

AU - Frisvad,Jens Christian

AU - Nielsen,Kristian Fog

PY - 2011

Y1 - 2011

N2 - Objectives: Pseudomonas aeruginosa is an opportunistic human pathogen, commonly infecting cystic fibrosis (CF) patients. Aspergilli, especially Aspergillus fumigatus, are also frequently isolated from CF patients. Our aim was to examine the possible interaction between P. aeruginosa and different Aspergillus species. Methods: A suspension of fungal spores was streaked onto WATM agar plates. After 24 hours incubation at 37 °C, a P. aeruginosa overnight culture was streaked out perpendicular to the fungal streak. The plates were incubated at 37 °C for five days, examined and plugs were extracted for HPLC-DAD and HPLC-DAD-MS analysis. Results: P. aeruginosa PAO1 suppressed growth of A. fumigatus, A. niger, A. flavus, A. oryzae, A. terreus and Emericella nidulans. HPLC and HPLC-DAD-MS results showed an increase in phenazine-1-carboxylic acid and phenazine-1-carboxamide production by P. aeruginosa in the contact area of A. niger, A. flavus, A. oryzae, but not A. fumigatus. In addition, other metabolites with UV chromophores similar to the phenazines were only found in the contact zone between Aspergillus and Pseudomonas. No change in secondary metabolite profiles were seen for the Aspergilli, when comparing with or without the presence of Pseudomonas. Conclusion: All Aspergilli tested, with the exception of A. fumigatus, triggered the upregulation of phenazine-1-carboxamide and phenazine-1-carboxylic acid production by P. aeruginosa. Surprisingly no changes in secondary metabolite profiles were detected in any of the Aspergilli.

AB - Objectives: Pseudomonas aeruginosa is an opportunistic human pathogen, commonly infecting cystic fibrosis (CF) patients. Aspergilli, especially Aspergillus fumigatus, are also frequently isolated from CF patients. Our aim was to examine the possible interaction between P. aeruginosa and different Aspergillus species. Methods: A suspension of fungal spores was streaked onto WATM agar plates. After 24 hours incubation at 37 °C, a P. aeruginosa overnight culture was streaked out perpendicular to the fungal streak. The plates were incubated at 37 °C for five days, examined and plugs were extracted for HPLC-DAD and HPLC-DAD-MS analysis. Results: P. aeruginosa PAO1 suppressed growth of A. fumigatus, A. niger, A. flavus, A. oryzae, A. terreus and Emericella nidulans. HPLC and HPLC-DAD-MS results showed an increase in phenazine-1-carboxylic acid and phenazine-1-carboxamide production by P. aeruginosa in the contact area of A. niger, A. flavus, A. oryzae, but not A. fumigatus. In addition, other metabolites with UV chromophores similar to the phenazines were only found in the contact zone between Aspergillus and Pseudomonas. No change in secondary metabolite profiles were seen for the Aspergilli, when comparing with or without the presence of Pseudomonas. Conclusion: All Aspergilli tested, with the exception of A. fumigatus, triggered the upregulation of phenazine-1-carboxamide and phenazine-1-carboxylic acid production by P. aeruginosa. Surprisingly no changes in secondary metabolite profiles were detected in any of the Aspergilli.

UR - http://www.eurobiofilms2011.ics.dk/

ER -