Ascaris Suum Infection Downregulates Inflammatory Pathways in the Pig Intestine In Vivo and in Human Dendritic Cells In Vitro.

Publication: Research - peer-reviewJournal article – Annual report year: 2018

DOI

  • Author: Midttun, Helene L. E.

    University of Copenhagen, Denmark

  • Author: Acevedo, Nathalie

    Universidad de Cartagena, Denmark

  • Author: Skallerup, Per

    University of Copenhagen, Denmark

  • Author: Almeida, Sara

    University of Copenhagen, Denmark

  • Author: Skovgaard, Kerstin

    Innate Immunology, Division of Immunology & Vaccinology, National Veterinary Institute, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Andresen, Lars

    University of Copenhagen, Denmark

  • Author: Skov, Søren

    University of Copenhagen, Denmark

  • Author: Caraballo, Luis

    Universidad de Cartagena, Colombia

  • Author: van Die, Irma

    VU University Medical Centre, Netherlands

  • Author: Jørgensen, Claus B.

    University of Copenhagen, Denmark

  • Author: Fredholm, Merete

    University of Copenhagen, Denmark

  • Author: Thamsborg, Stig M.

    University of Copenhagen, Denmark

  • Author: Nejsum, Peter

    Aarhus University, Denmark

  • Author: Williams, Andrew R.

    University of Copenhagen, Denmark

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Ascaris suum is a helminth parasite of pigs closely related to its human counterpart, A. lumbricoides, which infects almost 1 billion people. Ascaris is thought to modulate host immune and inflammatory responses, which may drive immune hyporesponsiveness during chronic infections. Using transcriptomic analysis, we show here that pigs with a chronic A. suum infection have a substantial suppression of inflammatory pathways in the intestinal mucosa, with a broad downregulation of genes encoding cytokines and antigen-processing and costimulatory molecules. A. suum body fluid (ABF) suppressed similar transcriptional pathways in human dendritic cells (DCs) in vitro. DCs exposed to ABF secreted minimal amounts of cytokines and had impaired production of cyclooxygengase-2, altered glucose metabolism, and reduced capacity to induce interferon-gamma production in T cells. Our in vivo and in vitro data provide an insight into mucosal immune modulation during Ascaris infection, and show that A. suum profoundly suppresses immune and inflammatory pathways
Original languageEnglish
JournalJournal of Infectious Diseases
Volume217
Issue number2
Pages (from-to)310-319
ISSN0022-1899
DOIs
StatePublished - 2018
CitationsWeb of Science® Times Cited: 3
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