Application of bipolar electrodialysis to E.coli fermentation for simultaneous acetate removal and pH control

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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The application of bipolar electrodialysis (BPED) for the simultaneous removal of inhibitory acetate and pH control during E. coli fermentation was investigated. A two cell pair electrodialysis module, consisting of cation exchange, anion exchange and bipolar membranes with working area of 100 cm2 each, was integrated with a standard 7 l stirred tank bioreactor. Results showed that BPED was beneficial in terms of in situ removal of inhibitory acetate and a reduction in the amount NH4OH used for pH control. In batch and fed-batch BPED fermentations, base additions were decreased by up to 50% in both cases compared to electrodialysis (ED) fermentations with pH controlled at 6.7 ± 0.1. Consequently, the final biomass (34.2 g DCW l−1) and recombinant protein (5.5 g l−1) concentrations obtained were increased by up to 37 and 20%, respectively.
Original languageEnglish
JournalBiotechnology Letters
Issue number8
Pages (from-to)1053-1057
StatePublished - 2010
CitationsWeb of Science® Times Cited: No match on DOI
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