Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection. / Boutrup, Torsten Snogdal; Schauser, Kirsten; Agerholm, Jørgen S; Jensen, Tim Kåre.

In: Acta Veterinaria Scandinavica (Online), Vol. 52, No. 17, 2010, p. 1 - 22.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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Boutrup, Torsten Snogdal; Schauser, Kirsten; Agerholm, Jørgen S; Jensen, Tim Kåre / Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection.

In: Acta Veterinaria Scandinavica (Online), Vol. 52, No. 17, 2010, p. 1 - 22.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Bibtex

@article{673af50eebef436aaf65b628273496d9,
title = "Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection",
publisher = "BioMed Central Ltd.",
author = "Boutrup, {Torsten Snogdal} and Kirsten Schauser and Agerholm, {Jørgen S} and Jensen, {Tim Kåre}",
note = "This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.",
year = "2010",
doi = "10.1186/1751-0147-52-17",
volume = "52",
number = "17",
pages = "1 -- 22",
journal = "Acta Veterinaria Scandinavica (Online)",
issn = "1751-0147",

}

RIS

TY - JOUR

T1 - Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection

A1 - Boutrup,Torsten Snogdal

A1 - Schauser,Kirsten

A1 - Agerholm,Jørgen S

A1 - Jensen,Tim Kåre

AU - Boutrup,Torsten Snogdal

AU - Schauser,Kirsten

AU - Agerholm,Jørgen S

AU - Jensen,Tim Kåre

PB - BioMed Central Ltd.

PY - 2010

Y1 - 2010

N2 - Background Porcine proliferative enteropathy in pigs is caused by the obligate, intracellular bacterium Lawsonia intracellularis. In vitro studies have shown close bacterium-cell interaction followed by cellular uptake of the bacterium within 3 h post inoculation (PI). However, knowledge of the initial in vivo interaction between porcine intestinal epithelium and the bacterium is limited. The aims of the present study were to evaluate the usefulness of a ligated small intestinal loop model to study L. intracellularis infections and to obtain information on the very early L. intracellularis-enterocyte interactions. Methods A ligated small intestinal loop model using three different L. intracellularis inocula was applied to 10- 11-week-old pigs. The inocula were 1) wild type bacteria derived from overnight incubation of L. intracellularis bacteria from spontaneous disease, 2) crude vaccine bacteria (Enterisol® Ileitis Vet), and 3) vaccine bacteria propagated in cell culture. The bacteria-enterocyte interaction was visualised using immunohistochemistry on specimens derived 1, 3 and 6 h PI respectively. Results Although at a low level, close contact between bacteria and the enterocyte brush border including intracellular uptake of bacteria in mature enterocytes was seen at 3 and 6 h PI for the vaccine and the propagated vaccine inocula. Interaction between the wild-type bacteria and villus enterocytes was scarce and only seen at 6 h PI, where a few bacteria were found in close contact with the brush border. Conclusions The ligated intestinal loop model was useful with respect to maintaining an intact intestinal morphology for up to 6 h. Furthermore, the study demonstrated that L. intracellularis interacts with villus enterocytes within 3 to 6 h after inoculation into intestinal loops and that the bacterium, as shown for the vaccine bacteria, propagated as well as non-propagated, was able to invade mature enterocytes. Thus, the study demonstrates the early intestinal invasion of L. intracellularis in vivo.

AB - Background Porcine proliferative enteropathy in pigs is caused by the obligate, intracellular bacterium Lawsonia intracellularis. In vitro studies have shown close bacterium-cell interaction followed by cellular uptake of the bacterium within 3 h post inoculation (PI). However, knowledge of the initial in vivo interaction between porcine intestinal epithelium and the bacterium is limited. The aims of the present study were to evaluate the usefulness of a ligated small intestinal loop model to study L. intracellularis infections and to obtain information on the very early L. intracellularis-enterocyte interactions. Methods A ligated small intestinal loop model using three different L. intracellularis inocula was applied to 10- 11-week-old pigs. The inocula were 1) wild type bacteria derived from overnight incubation of L. intracellularis bacteria from spontaneous disease, 2) crude vaccine bacteria (Enterisol® Ileitis Vet), and 3) vaccine bacteria propagated in cell culture. The bacteria-enterocyte interaction was visualised using immunohistochemistry on specimens derived 1, 3 and 6 h PI respectively. Results Although at a low level, close contact between bacteria and the enterocyte brush border including intracellular uptake of bacteria in mature enterocytes was seen at 3 and 6 h PI for the vaccine and the propagated vaccine inocula. Interaction between the wild-type bacteria and villus enterocytes was scarce and only seen at 6 h PI, where a few bacteria were found in close contact with the brush border. Conclusions The ligated intestinal loop model was useful with respect to maintaining an intact intestinal morphology for up to 6 h. Furthermore, the study demonstrated that L. intracellularis interacts with villus enterocytes within 3 to 6 h after inoculation into intestinal loops and that the bacterium, as shown for the vaccine bacteria, propagated as well as non-propagated, was able to invade mature enterocytes. Thus, the study demonstrates the early intestinal invasion of L. intracellularis in vivo.

U2 - 10.1186/1751-0147-52-17

DO - 10.1186/1751-0147-52-17

JO - Acta Veterinaria Scandinavica (Online)

JF - Acta Veterinaria Scandinavica (Online)

SN - 1751-0147

IS - 17

VL - 52

SP - 1

EP - 22

ER -