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Antimicrobial-resistant faecal Escherichia coli in wild mammals in central Europe: multiresistant Escherichia coli producing extended-spectrum ß-lactamases in wild boars. / Literak, I.; Dolejska, Monika; Radimersky, T.; Klimes, J.; Friedman, M.; Aarestrup, Frank Møller; Hasman, Henrik; Cizek, A.

In: Journal of Applied Microbiology, Vol. 108, No. 5, 2010, p. 1702-1711.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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Literak, I.; Dolejska, Monika; Radimersky, T.; Klimes, J.; Friedman, M.; Aarestrup, Frank Møller; Hasman, Henrik; Cizek, A. / Antimicrobial-resistant faecal Escherichia coli in wild mammals in central Europe: multiresistant Escherichia coli producing extended-spectrum ß-lactamases in wild boars.

In: Journal of Applied Microbiology, Vol. 108, No. 5, 2010, p. 1702-1711.

Publication: Research - peer-reviewJournal article – Annual report year: 2010

Bibtex

@article{89a74194d48949479bf6b12713bf7875,
title = "Antimicrobial-resistant faecal Escherichia coli in wild mammals in central Europe: multiresistant Escherichia coli producing extended-spectrum ß-lactamases in wild boars",
keywords = "Czech Republic, Slovakia, Sus scrofa, antibiotic resistance, intestinal bacteria",
publisher = "Wiley-Blackwell Publishing Ltd.",
author = "I. Literak and Monika Dolejska and T. Radimersky and J. Klimes and M. Friedman and Aarestrup, {Frank Møller} and Henrik Hasman and A. Cizek",
year = "2010",
doi = "10.1111/j.1365-2672.2009.04572.x",
volume = "108",
number = "5",
pages = "1702--1711",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",

}

RIS

TY - JOUR

T1 - Antimicrobial-resistant faecal Escherichia coli in wild mammals in central Europe: multiresistant Escherichia coli producing extended-spectrum ß-lactamases in wild boars

A1 - Literak,I.

A1 - Dolejska,Monika

A1 - Radimersky,T.

A1 - Klimes,J.

A1 - Friedman,M.

A1 - Aarestrup,Frank Møller

A1 - Hasman,Henrik

A1 - Cizek,A.

AU - Literak,I.

AU - Dolejska,Monika

AU - Radimersky,T.

AU - Klimes,J.

AU - Friedman,M.

AU - Aarestrup,Frank Møller

AU - Hasman,Henrik

AU - Cizek,A.

PB - Wiley-Blackwell Publishing Ltd.

PY - 2010

Y1 - 2010

N2 - Aims: To determine the presence of antibiotic-resistant faecal Escherichia coli in populations of wild mammals in the Czech Republic and Slovakia. Methods and Results: Rectal swabs or faeces collected during 2006-2008 from wild mammals were spread on MacConkey agar and MacConkey agar containing 2 mg l-1 of cefotaxime. From plates with positive growth, one isolate was recovered and identified as E. coli. Susceptibility to 12 antibiotics was tested using the disk diffusion method. Resistance genes, class 1 and 2 integrons and gene cassettes were detected in resistant isolates by polymerase chain reaction (PCR). Extended-spectrum beta-lactamases (ESBL) were further characterized by DNA sequencing, macrorestriction profiling and determination of plasmid sizes. Plasmid DNA was subjected to EcoRV digestion, transferability by conjugation and incompatibility grouping by multiplex PCR. The prevalence of resistant isolates was 2% in small terrestrial mammals (rodents and insectivores, n(E. coli) = 242), 12% in wild ruminants and foxes (n(E. coli) = 42), while no resistant isolates were detected in brown bears (n(E. coli) = 16). In wild boars (Sus scrofa) (n(E. coli) = 290), the prevalence of resistant isolates was 6%. Class 1 and 2 integrons with various gene cassettes were recorded in resistant isolates. From wild boars, five (2%, n(rectal smears) = 293) multiresistant isolates producing ESBL were recovered: one isolate with bla(CTX-M-1) + bla(TEM-1), three with bla(CTX-M-1) and one with bla(TEM-52b). The bla(CTX-M-1) genes were carried on approx. 90 kb IncI1 conjugative plasmids. Conclusions: Antibiotic-resistant E. coli occured in populations of wild mammals in various prevalences. Significance and Impact of the Study: Wild mammals are reservoirs of antibiotic-resistant E. coli including ESBL-producing strains which were found in wild boars.

AB - Aims: To determine the presence of antibiotic-resistant faecal Escherichia coli in populations of wild mammals in the Czech Republic and Slovakia. Methods and Results: Rectal swabs or faeces collected during 2006-2008 from wild mammals were spread on MacConkey agar and MacConkey agar containing 2 mg l-1 of cefotaxime. From plates with positive growth, one isolate was recovered and identified as E. coli. Susceptibility to 12 antibiotics was tested using the disk diffusion method. Resistance genes, class 1 and 2 integrons and gene cassettes were detected in resistant isolates by polymerase chain reaction (PCR). Extended-spectrum beta-lactamases (ESBL) were further characterized by DNA sequencing, macrorestriction profiling and determination of plasmid sizes. Plasmid DNA was subjected to EcoRV digestion, transferability by conjugation and incompatibility grouping by multiplex PCR. The prevalence of resistant isolates was 2% in small terrestrial mammals (rodents and insectivores, n(E. coli) = 242), 12% in wild ruminants and foxes (n(E. coli) = 42), while no resistant isolates were detected in brown bears (n(E. coli) = 16). In wild boars (Sus scrofa) (n(E. coli) = 290), the prevalence of resistant isolates was 6%. Class 1 and 2 integrons with various gene cassettes were recorded in resistant isolates. From wild boars, five (2%, n(rectal smears) = 293) multiresistant isolates producing ESBL were recovered: one isolate with bla(CTX-M-1) + bla(TEM-1), three with bla(CTX-M-1) and one with bla(TEM-52b). The bla(CTX-M-1) genes were carried on approx. 90 kb IncI1 conjugative plasmids. Conclusions: Antibiotic-resistant E. coli occured in populations of wild mammals in various prevalences. Significance and Impact of the Study: Wild mammals are reservoirs of antibiotic-resistant E. coli including ESBL-producing strains which were found in wild boars.

KW - Czech Republic

KW - Slovakia

KW - Sus scrofa

KW - antibiotic resistance

KW - intestinal bacteria

U2 - 10.1111/j.1365-2672.2009.04572.x

DO - 10.1111/j.1365-2672.2009.04572.x

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 5

VL - 108

SP - 1702

EP - 1711

ER -