Antibiotic tolerance and microbial biofilms

Research output: ResearchSound/Visual production (digital) – Annual report year: 2009

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Increased tolerance to antimicrobial agents is thought to be an important feature of microbes growing in biofilms. We study the dynamics of antibiotic action within hydrodynamic flow chamber biofilms of Escherichia coli and Pseudomonas aeruginosa using isogenic mutants and fluorescent gene expression reporters and we address the question of how biofilm organization affects antibiotic susceptibility. The dynamics of microbial killing is monitored by viable count determination, and confocal laser microscopy. Our work shows that the apparent increased antibiotic tolerance is due to the formation of antibiotic tolerant subpopulations within the biofilm. The formation of these subpopulations is highly variable and dependent on the antibiotic used, the biofilm structural organization and the induction of specific tolerance mechanisms.
Original languageEnglish
Publication date2009
StatePublished - 2009
EventCentral European Symposium on Antimicrobial Resistance - Zadar, Croatia
Duration: 23 Sep 200926 Sep 2009
http://www.hmd-cms.hr/cesar2009/programme.html

Conference

ConferenceCentral European Symposium on Antimicrobial Resistance
CountryCroatia
CityZadar
Period23/09/200926/09/2009
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