• Author: Nielsen, H. S.

  • Author: Storgaard, Torben

  • Author: Oleksiewicz, M.B.

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A rapid method was developed for partial characterization of the replicase-encoding open reading frame 1 (ORF 1) of porcine reproductive and respiratory syndrome virus (PRRSV). It comprised long RT-PCR amplification of 11.1 kb (94%) of ORF 1, followed by restriction fragment length polymorphism analysis. The method was used to compare ORF 1 sequences of two divergent European-type PRRSV strains. Our results indicated that the structural and replicase parts of these two strains had evolved at overall similar rates.
Original languageEnglish
JournalVeterinary Microbiology
Publication date2000
Volume76
Issue3
Pages221-228
ISSN0378-1135
StatePublished

Keywords

  • replicase gene, long RT-PCR, porcine reproductive and respiratory syndrome virus, RFLP
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