Analysis of laser-induced heating in optical neuronal guidance

Publication: Research - peer-reviewJournal article – Annual report year: 2012

View graph of relations

Recently, it has been shown that it is possible to control the growth direction of neuronal growth cones by stimulation with weak laser light; an effect dubbed optical neuronal guidance. The effect exists for a broad range of laser wavelengths, spot sizes, spot intensities, optical intensity profiles and beam modulations, but it is unknown which biophysical mechanisms govern it. Based on thermodynamic modeling and simulation using published experimental parameters as input, we argue that the guidance is linked to heating. Until now, temperature effects due to laser-induced heating of the guided neuron have been neglected in the optical neuronal guidance literature. The results of our finite-element-method simulations show the relevance of the temperature field in optical guidance experiments and are consistent with published experimental results and modeling in the field of optical traps. Furthermore, we propose two experiments designed to test this hypotheses experimentally. For one of these experiments, we have designed a microfluidic platform, to be made using standard microfabrication techniques, for incubation of neurons in temperature gradients on micrometer lengthscales.
Original languageEnglish
JournalJournal of Neuroscience Methods
Issue number1
Pages (from-to)168-177
StatePublished - 2012
CitationsWeb of Science® Times Cited: 15


  • Optical neuronal guidance, Neuronal growth, Laser heating, Temperature gradient, Microfluidics, Biochip
Download as:
Download as PDF
Select render style:
Download as HTML
Select render style:
Download as Word
Select render style:

Download statistics

No data available

ID: 10084500