Analysis of flux estimates based on C-13-labelling experiments

Publication: Research - peer-reviewJournal article – Annual report year: 2002

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Analysis of flux estimates based on C-13-labelling experiments. / Christensen, Bjarke; Gombert, Andreas Karoly; Nielsen, Jens.

In: European Journal of Biochemistry, Vol. 269, No. 11, 2002, p. 2795-2800.

Publication: Research - peer-reviewJournal article – Annual report year: 2002

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Author

Christensen, Bjarke; Gombert, Andreas Karoly; Nielsen, Jens / Analysis of flux estimates based on C-13-labelling experiments.

In: European Journal of Biochemistry, Vol. 269, No. 11, 2002, p. 2795-2800.

Publication: Research - peer-reviewJournal article – Annual report year: 2002

Bibtex

@article{edb42e5534b143dcb779f99bcf27e2a6,
title = "Analysis of flux estimates based on C-13-labelling experiments",
publisher = "Springer Verlag",
author = "Bjarke Christensen and Gombert, {Andreas Karoly} and Jens Nielsen",
year = "2002",
volume = "269",
number = "11",
pages = "2795--2800",
journal = "European Journal of Biochemistry",
issn = "0014-2956",

}

RIS

TY - JOUR

T1 - Analysis of flux estimates based on C-13-labelling experiments

A1 - Christensen,Bjarke

A1 - Gombert,Andreas Karoly

A1 - Nielsen,Jens

AU - Christensen,Bjarke

AU - Gombert,Andreas Karoly

AU - Nielsen,Jens

PB - Springer Verlag

PY - 2002

Y1 - 2002

N2 - Modelling of the fluxes in central metabolism can be performed by combining labelling experiments with metabolite balancing. Using this approach, multiple samples from a cultivation of Saccharomyces cerevisiae in metabolic and isotopic steady state were analysed, and the metabolic fluxes in central metabolism were estimated. In the various samples, the estimates of the central metabolic pathways, the tricarboxylic acid cycle, the oxidative pentose phosphate pathway and the anaplerotic pathway, showed an unprecedented reproducibility. The high reproducibility was obtained with fractional labellings of individual carbon atoms as the calculational base, illustrating that the more complex modelling using isotopomers is not necessarily superior with respect to reproducibility of the flux estimates. Based on these results some general difficulties in flux estimation are discussed.

AB - Modelling of the fluxes in central metabolism can be performed by combining labelling experiments with metabolite balancing. Using this approach, multiple samples from a cultivation of Saccharomyces cerevisiae in metabolic and isotopic steady state were analysed, and the metabolic fluxes in central metabolism were estimated. In the various samples, the estimates of the central metabolic pathways, the tricarboxylic acid cycle, the oxidative pentose phosphate pathway and the anaplerotic pathway, showed an unprecedented reproducibility. The high reproducibility was obtained with fractional labellings of individual carbon atoms as the calculational base, illustrating that the more complex modelling using isotopomers is not necessarily superior with respect to reproducibility of the flux estimates. Based on these results some general difficulties in flux estimation are discussed.

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

SN - 0014-2956

IS - 11

VL - 269

SP - 2795

EP - 2800

ER -