An automated and highly efficient method for counting and measuring fluorescent foci in rod-shaped bacteria

Publication: Research - peer-reviewJournal article – Annual report year: 2010

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P>Direct measurements of cells from photo micrographs are becoming increasingly used when investigating the position and/or distribution of chromosomal loci in bacteria. In general, these measurements have been done manually, and without clear definition of how they are made. Here we present a procedure for standardizing the measurement of cell properties from phase contrast images. Furthermore, we present a program using these standardized methods that can measure the intracellular positions of fluorescent foci in bacterial cells faster and with more precision than manual measurement.
Original languageEnglish
JournalJournal of Microscopy
Publication date2010
Volume239
Issue3
Pages194-199
ISSN0022-2720
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 2

Keywords

  • Automatic analysis of bacterial properties, fluorescent chromosome locations, chromosome segregation
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