Amplification and direct sequence analysis of the 23S rRNA gene from thermophilic bacteria

Publication: Research - peer-reviewJournal article – Annual report year: 2001

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We present a simplified and fast method to obtain high-quality sequences directly from PCRs without the traditional gel purification. We also report on an improved method to obtain sequence-quality PCR products from microorganisms that are difficult to lyse with no need for DNA extraction. The technique uses exonuclease I and shrimp alkaline phosphatase to degrade residual dNTPs and primers. Our technique is shown to work on both Gram-positive and Gram-negative bacteria
Original languageEnglish
JournalBioTechniques
Publication date2001
Volume30
Issue2
Pages414-420
ISSN0736-6205
StatePublished
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