TY - JOUR

T1 - Advanced microtechnologies for detection of chromosome abnormalities by fluorescent in situ hybridization.

A1 - Kwasny,Dorota

A1 - Vedarethinam,Indumathi

A1 - Shah,Pranjul

A1 - Dimaki,Maria

A1 - Silahtaroglu,Asli

A1 - Tumer,Zeynep

A1 - Svendsen,Winnie Edith

AU - Kwasny,Dorota

AU - Vedarethinam,Indumathi

AU - Shah,Pranjul

AU - Dimaki,Maria

AU - Silahtaroglu,Asli

AU - Tumer,Zeynep

AU - Svendsen,Winnie Edith

PB - Springer New York LLC

PY - 2012

Y1 - 2012

N2 - Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cytogenetic techniques such as fluorescent in situ hybridization (FISH). To improve FISH application in cytogenetic analysis the issues with long experimental time, high volumes of expensive reagents and requirement for trained technicians need to be addressed. The protocol has recently evolved towards on chip detection of chromosome abnormalities with the development of microsystems for FISH analysis. The challenges addressed by the developed microsystems are mainly the automation of the assay performance, reduction in probe volume, as well as reduction of assay time. The recent focus on the development of automated systems for performing FISH on chip is summarized in this review.

AB - Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cytogenetic techniques such as fluorescent in situ hybridization (FISH). To improve FISH application in cytogenetic analysis the issues with long experimental time, high volumes of expensive reagents and requirement for trained technicians need to be addressed. The protocol has recently evolved towards on chip detection of chromosome abnormalities with the development of microsystems for FISH analysis. The challenges addressed by the developed microsystems are mainly the automation of the assay performance, reduction in probe volume, as well as reduction of assay time. The recent focus on the development of automated systems for performing FISH on chip is summarized in this review.

KW - Fluorescent in situ hybridization

KW - FISH on chip

KW - Microsystems

KW - Cytogenetic analysis

U2 - 10.1007/s10544-011-9622-7

DO - 10.1007/s10544-011-9622-7

JO - Biomedical Microdevices

JF - Biomedical Microdevices

SN - 1387-2176

IS - 3

VL - 14

SP - 453

EP - 460

ER -