Advanced Microscopy of Microbial Cells

Publication: Research - peer-reviewBook chapter – Annual report year: 2011

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Growing awareness of heterogeneity in cells of microbial populations has emphasized the importance of advanced microscopy for visualization and understanding of the molecular mechanisms underlying cell-to-cell variation. In this review, we highlight some of the recent advances in confocal microscopy, super-resolution optical microscopy (STED, SIM, PALM) as well as atomic force microscopy and Raman spectroscopy. Using examples of bistability in microbial populations as well as biofilm development and differentiation in bacterial and yeast consortia, we demonstrate the importance of microscopy for visualization of variation between cells in phenotypic traits such as gene expression.
Original languageEnglish
TitleAdvances in Biochemical Engineering/Biotechnology
Volume124
PublisherSpringer
Publication date2011
Pages21-54
ISBN (print)978-3-642-16886-4
ISBN (electronic)978-3-642-16887-1
StatePublished
NameAdvances in Biochemical Engineering/Biotechnology
ISSN (Print)0724-6145

Keywords

  • Bacteria, Bistability, Advanced microscopy techniques, Single-cell gene expression, Biofilm development and differentiation, Yeast
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