Actinobacillus pleruropneumoniae transcriptome analysis during early infection - coping with a hostile environment

Publication: Research - peer-reviewConference abstract in proceedings – Annual report year: 2011

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Actinobacillus pleruropneumoniae transcriptome analysis during early infection - coping with a hostile environment. / Schou, Kirstine Klitgaard; Rundsten, Carsten Friis; Jensen, Tim Kåre; Angen, Øystein; Boye, Mette.

International Pasteurellaceae Conference 2011: Final Programme and Abstracts. 2011. p. P52.

Publication: Research - peer-reviewConference abstract in proceedings – Annual report year: 2011

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Schou, Kirstine Klitgaard; Rundsten, Carsten Friis; Jensen, Tim Kåre; Angen, Øystein; Boye, Mette / Actinobacillus pleruropneumoniae transcriptome analysis during early infection - coping with a hostile environment.

International Pasteurellaceae Conference 2011: Final Programme and Abstracts. 2011. p. P52.

Publication: Research - peer-reviewConference abstract in proceedings – Annual report year: 2011

Bibtex

@inbook{2b84d84a1e214ae2990eaea588b16499,
title = "Actinobacillus pleruropneumoniae transcriptome analysis during early infection - coping with a hostile environment",
author = "Schou, {Kirstine Klitgaard} and Rundsten, {Carsten Friis} and Jensen, {Tim Kåre} and Øystein Angen and Mette Boye",
year = "2011",
pages = "P52",
booktitle = "International Pasteurellaceae Conference 2011",

}

RIS

TY - ABST

T1 - Actinobacillus pleruropneumoniae transcriptome analysis during early infection - coping with a hostile environment

A1 - Schou,Kirstine Klitgaard

A1 - Rundsten,Carsten Friis

A1 - Jensen,Tim Kåre

A1 - Angen,Øystein

A1 - Boye,Mette

AU - Schou,Kirstine Klitgaard

AU - Rundsten,Carsten Friis

AU - Jensen,Tim Kåre

AU - Angen,Øystein

AU - Boye,Mette

PY - 2011

Y1 - 2011

N2 - Aim: To obtain an increased understanding of how the porcine lung pathogen Actinobacillus pleuropneumoniae (Ap) establish infection in the host. Understanding the means by which a pathogen establishes and maintains infection in the host organism is the first step towards controlling disease. Methods: The local in vivo genetic response of Ap during the early phase of infection in porcine lungs was detailed using pangenomic microarray analysis. The global transcriptional patterns of Ap serotype 2 and 6 isolated from lung tissue biopsies of 25 experimentally infected pigs were compared at four time points between 6 and 48 hours post infection. Results: We identified 310 genes (p <1.0 × 10-8) that were differentially expressed during the first 48 hours of infection. Most of these genes appeared to be up-regulated at 6 hours post inoculation after which the expression gradually declined over the next 42 hours. Functional analysis identified a number of putative virulence genes to be initially up-regulated. Conclusions: This is the first study monitoring the development of Ap response in the porcine host during early infection. The ability of pathogenic bacteria to adjust gene expression in response to environmental stimuli is critical for bacterial survival within the host. The genes identified as differentially expressed in this study may represent a core set of genes which are mobilized to cope with the host immune response and adapt to the hostile environment. The potential virulence genes identified may represent valuable candidates for vaccine development.

AB - Aim: To obtain an increased understanding of how the porcine lung pathogen Actinobacillus pleuropneumoniae (Ap) establish infection in the host. Understanding the means by which a pathogen establishes and maintains infection in the host organism is the first step towards controlling disease. Methods: The local in vivo genetic response of Ap during the early phase of infection in porcine lungs was detailed using pangenomic microarray analysis. The global transcriptional patterns of Ap serotype 2 and 6 isolated from lung tissue biopsies of 25 experimentally infected pigs were compared at four time points between 6 and 48 hours post infection. Results: We identified 310 genes (p <1.0 × 10-8) that were differentially expressed during the first 48 hours of infection. Most of these genes appeared to be up-regulated at 6 hours post inoculation after which the expression gradually declined over the next 42 hours. Functional analysis identified a number of putative virulence genes to be initially up-regulated. Conclusions: This is the first study monitoring the development of Ap response in the porcine host during early infection. The ability of pathogenic bacteria to adjust gene expression in response to environmental stimuli is critical for bacterial survival within the host. The genes identified as differentially expressed in this study may represent a core set of genes which are mobilized to cope with the host immune response and adapt to the hostile environment. The potential virulence genes identified may represent valuable candidates for vaccine development.

UR - http://www.pasteurellaceae.life.ku.dk/~/media/Pasteurellaceae/IPC_Programme_WEB_LOW.ashx

BT - International Pasteurellaceae Conference 2011

T2 - International Pasteurellaceae Conference 2011

SP - P52

ER -