A structural analysis of M protein in coronavirus assembly and morphology

Publication: Research - peer-reviewJournal article – Annual report year: 2011

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A structural analysis of M protein in coronavirus assembly and morphology. / Neuman, Benjamin W.; Kiss, Gabriella; Kunding, Andreas Hjarne; Bhella, David; Baksh, M. Fazil; Connelly, Stephen; Droese, Ben; Klaus, Joseph P.; Makino, Shinji; Sawicki, Stanley G.; Siddell, Stuart G.; Stamou, Dimitrios G.; Wilson, Ian A.; Kuhn, Peter; Buchmeier, Michael J.

In: Journal of Structural Biology, Vol. 174, 2011, p. 11-22.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Harvard

Neuman, BW, Kiss, G, Kunding, AH, Bhella, D, Baksh, MF, Connelly, S, Droese, B, Klaus, JP, Makino, S, Sawicki, SG, Siddell, SG, Stamou, DG, Wilson, IA, Kuhn, P & Buchmeier, MJ 2011, 'A structural analysis of M protein in coronavirus assembly and morphology' Journal of Structural Biology, vol 174, pp. 11-22., 10.1016/j.jsb.2010.11.021

APA

Neuman, B. W., Kiss, G., Kunding, A. H., Bhella, D., Baksh, M. F., Connelly, S., ... Buchmeier, M. J. (2011). A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology, 174, 11-22. 10.1016/j.jsb.2010.11.021

CBE

Neuman BW, Kiss G, Kunding AH, Bhella D, Baksh MF, Connelly S, Droese B, Klaus JP, Makino S, Sawicki SG, Siddell SG, Stamou DG, Wilson IA, Kuhn P, Buchmeier MJ. 2011. A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology. 174:11-22. Available from: 10.1016/j.jsb.2010.11.021

MLA

Vancouver

Neuman BW, Kiss G, Kunding AH, Bhella D, Baksh MF, Connelly S et al. A structural analysis of M protein in coronavirus assembly and morphology. Journal of Structural Biology. 2011;174:11-22. Available from: 10.1016/j.jsb.2010.11.021

Author

Neuman, Benjamin W.; Kiss, Gabriella; Kunding, Andreas Hjarne; Bhella, David; Baksh, M. Fazil; Connelly, Stephen; Droese, Ben; Klaus, Joseph P.; Makino, Shinji; Sawicki, Stanley G.; Siddell, Stuart G.; Stamou, Dimitrios G.; Wilson, Ian A.; Kuhn, Peter; Buchmeier, Michael J. / A structural analysis of M protein in coronavirus assembly and morphology.

In: Journal of Structural Biology, Vol. 174, 2011, p. 11-22.

Publication: Research - peer-reviewJournal article – Annual report year: 2011

Bibtex

@article{2aa56be777f2463299e17b5c364e674c,
title = "A structural analysis of M protein in coronavirus assembly and morphology",
publisher = "Academic Press",
author = "Neuman, {Benjamin W.} and Gabriella Kiss and Kunding, {Andreas Hjarne} and David Bhella and Baksh, {M. Fazil} and Stephen Connelly and Ben Droese and Klaus, {Joseph P.} and Shinji Makino and Sawicki, {Stanley G.} and Siddell, {Stuart G.} and Stamou, {Dimitrios G.} and Wilson, {Ian A.} and Peter Kuhn and Buchmeier, {Michael J.}",
year = "2011",
doi = "10.1016/j.jsb.2010.11.021",
volume = "174",
pages = "11--22",
journal = "Journal of Structural Biology",
issn = "1047-8477",

}

RIS

TY - JOUR

T1 - A structural analysis of M protein in coronavirus assembly and morphology

A1 - Neuman,Benjamin W.

A1 - Kiss,Gabriella

A1 - Kunding,Andreas Hjarne

A1 - Bhella,David

A1 - Baksh,M. Fazil

A1 - Connelly,Stephen

A1 - Droese,Ben

A1 - Klaus,Joseph P.

A1 - Makino,Shinji

A1 - Sawicki,Stanley G.

A1 - Siddell,Stuart G.

A1 - Stamou,Dimitrios G.

A1 - Wilson,Ian A.

A1 - Kuhn,Peter

A1 - Buchmeier,Michael J.

AU - Neuman,Benjamin W.

AU - Kiss,Gabriella

AU - Kunding,Andreas Hjarne

AU - Bhella,David

AU - Baksh,M. Fazil

AU - Connelly,Stephen

AU - Droese,Ben

AU - Klaus,Joseph P.

AU - Makino,Shinji

AU - Sawicki,Stanley G.

AU - Siddell,Stuart G.

AU - Stamou,Dimitrios G.

AU - Wilson,Ian A.

AU - Kuhn,Peter

AU - Buchmeier,Michael J.

PB - Academic Press

PY - 2011

Y1 - 2011

N2 - The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.<br/> Keyword: Cryo-electron microscopy,Viral matrix protein,Coronavirus,Pleomorphic virus structure,Cryo-electron tomography

AB - The M protein of coronavirus plays a central role in virus assembly, turning cellular membranes into workshops where virus and host factors come together to make new virus particles. We investigated how M structure and organization is related to virus shape and size using cryo-electron microscopy, tomography and statistical analysis. We present evidence that suggests M can adopt two conformations and that membrane curvature is regulated by one M conformer. Elongated M protein is associated with rigidity, clusters of spikes and a relatively narrow range of membrane curvature. In contrast, compact M protein is associated with flexibility and low spike density. Analysis of several types of virus-like particles and virions revealed that S protein, N protein and genomic RNA each help to regulate virion size and variation, presumably through interactions with M. These findings provide insight into how M protein functions to promote virus assembly.<br/> Keyword: Cryo-electron microscopy,Viral matrix protein,Coronavirus,Pleomorphic virus structure,Cryo-electron tomography

U2 - 10.1016/j.jsb.2010.11.021

DO - 10.1016/j.jsb.2010.11.021

JO - Journal of Structural Biology

JF - Journal of Structural Biology

SN - 1047-8477

VL - 174

SP - 11

EP - 22

ER -