A pig model of acute Staphylococcus aureus induced pyemia

Publication: Research - peer-reviewJournal article – Annual report year: 2009

Standard

A pig model of acute Staphylococcus aureus induced pyemia. / Nielsen, O. L.; Iburg, T.; Aalbæk, B.; Leifsson, P. S.; Agerholm, J. S.; Heegaard, Peter M. H.; Boye, Mette; Simon, S.; Jensen, K. B.; Christensen, S.; Melsen, K.; Bak, A. K.; Backman, E. R.; Jørgensen, M. H.; Groegler, D.K.; Jensen, A. L.; Kjelgaard-Hansen, M.; Jensen, H. E.

In: Acta Veterinaria Scandinavica (Online Edition), Vol. 51, 2009, p. 14.

Publication: Research - peer-reviewJournal article – Annual report year: 2009

Harvard

Nielsen, OL, Iburg, T, Aalbæk, B, Leifsson, PS, Agerholm, JS, Heegaard, PMH, Boye, M, Simon, S, Jensen, KB, Christensen, S, Melsen, K, Bak, AK, Backman, ER, Jørgensen, MH, Groegler, DK, Jensen, AL, Kjelgaard-Hansen, M & Jensen, HE 2009, 'A pig model of acute Staphylococcus aureus induced pyemia' Acta Veterinaria Scandinavica (Online Edition), vol 51, pp. 14., 10.1186/1751-0147-51-14

APA

Nielsen, O. L., Iburg, T., Aalbæk, B., Leifsson, P. S., Agerholm, J. S., Heegaard, P. M. H., ... Jensen, H. E. (2009). A pig model of acute Staphylococcus aureus induced pyemia. Acta Veterinaria Scandinavica (Online Edition), 51, 14. 10.1186/1751-0147-51-14

CBE

Nielsen OL, Iburg T, Aalbæk B, Leifsson PS, Agerholm JS, Heegaard PMH, Boye M, Simon S, Jensen KB, Christensen S, Melsen K, Bak AK, Backman ER, Jørgensen MH, Groegler DK, Jensen AL, Kjelgaard-Hansen M, Jensen HE. 2009. A pig model of acute Staphylococcus aureus induced pyemia. Acta Veterinaria Scandinavica (Online Edition). 51:14. Available from: 10.1186/1751-0147-51-14

MLA

Vancouver

Nielsen OL, Iburg T, Aalbæk B, Leifsson PS, Agerholm JS, Heegaard PMH et al. A pig model of acute Staphylococcus aureus induced pyemia. Acta Veterinaria Scandinavica (Online Edition). 2009;51:14. Available from: 10.1186/1751-0147-51-14

Author

Nielsen, O. L.; Iburg, T.; Aalbæk, B.; Leifsson, P. S.; Agerholm, J. S.; Heegaard, Peter M. H.; Boye, Mette; Simon, S.; Jensen, K. B.; Christensen, S.; Melsen, K.; Bak, A. K.; Backman, E. R.; Jørgensen, M. H.; Groegler, D.K.; Jensen, A. L.; Kjelgaard-Hansen, M.; Jensen, H. E. / A pig model of acute Staphylococcus aureus induced pyemia.

In: Acta Veterinaria Scandinavica (Online Edition), Vol. 51, 2009, p. 14.

Publication: Research - peer-reviewJournal article – Annual report year: 2009

Bibtex

@article{23fef3c0e7824e61942df9a99410cb76,
title = "A pig model of acute Staphylococcus aureus induced pyemia",
publisher = "BioMed Central Ltd.",
author = "Nielsen, {O. L.} and T. Iburg and B. Aalbæk and Leifsson, {P. S.} and Agerholm, {J. S.} and Heegaard, {Peter M. H.} and Mette Boye and S. Simon and Jensen, {K. B.} and S. Christensen and K. Melsen and Bak, {A. K.} and Backman, {E. R.} and Jørgensen, {M. H.} and D.K. Groegler and Jensen, {A. L.} and M. Kjelgaard-Hansen and Jensen, {H. E.}",
year = "2009",
doi = "10.1186/1751-0147-51-14",
volume = "51",
pages = "14",
journal = "Acta Veterinaria Scandinavica (Online Edition)",
issn = "1751-0147",

}

RIS

TY - JOUR

T1 - A pig model of acute Staphylococcus aureus induced pyemia

A1 - Nielsen,O. L.

A1 - Iburg,T.

A1 - Aalbæk,B.

A1 - Leifsson,P. S.

A1 - Agerholm,J. S.

A1 - Heegaard,Peter M. H.

A1 - Boye,Mette

A1 - Simon,S.

A1 - Jensen,K. B.

A1 - Christensen,S.

A1 - Melsen,K.

A1 - Bak,A. K.

A1 - Backman,E. R.

A1 - Jørgensen,M. H.

A1 - Groegler,D.K.

A1 - Jensen,A. L.

A1 - Kjelgaard-Hansen,M.

A1 - Jensen,H. E.

AU - Nielsen,O. L.

AU - Iburg,T.

AU - Aalbæk,B.

AU - Leifsson,P. S.

AU - Agerholm,J. S.

AU - Heegaard,Peter M. H.

AU - Boye,Mette

AU - Simon,S.

AU - Jensen,K. B.

AU - Christensen,S.

AU - Melsen,K.

AU - Bak,A. K.

AU - Backman,E. R.

AU - Jørgensen,M. H.

AU - Groegler,D.K.

AU - Jensen,A. L.

AU - Kjelgaard-Hansen,M.

AU - Jensen,H. E.

PB - BioMed Central Ltd.

PY - 2009

Y1 - 2009

N2 - Background: Sepsis caused by Staphylococcus aureus constitutes an important cause of morbidity and mortality in humans, and the incidence of this disease-entity is increasing. In this paper we describe the initial microbial dynamics and lesions in pigs experimentally infected with S. aureus, with the aim of mimicking human sepsis and pyemia. Methods: The study was conducted in anaesthetized and intravenously inoculated pigs, and was based on bacteriological examination of blood and testing of blood for IL-6 and C-reactive protein. Following killing of the animals and necropsy bacteriological and histological examinations of different organs were performed 4, 5 or 6 h after inoculation. Results: Clearance of bacteria from the blood was completed within the first 2 h in some of the pigs and the highest bacterial load was recorded in the lungs as compared to the spleen, liver and bones. This probably was a consequence of both the intravenous route of inoculation and the presence of pulmonary intravascular macrophages. Inoculation of bacteria induced formation of acute microabscesses in the lungs, spleen and liver, but not in the kidneys or bones. No generalized inflammatory response was recorded, i.e. IL-6 was not detected in the blood and C-reactive protein did not increase, probably because of the short time course of the study. Conclusion: This study demonstrates the successful induction of acute pyemia (microabscesses), and forms a basis for future experiments that should include inoculation with strains of S. aureus isolated from man and an extension of the timeframe aiming at inducing sepsis, severe sepsis and septic shock.

AB - Background: Sepsis caused by Staphylococcus aureus constitutes an important cause of morbidity and mortality in humans, and the incidence of this disease-entity is increasing. In this paper we describe the initial microbial dynamics and lesions in pigs experimentally infected with S. aureus, with the aim of mimicking human sepsis and pyemia. Methods: The study was conducted in anaesthetized and intravenously inoculated pigs, and was based on bacteriological examination of blood and testing of blood for IL-6 and C-reactive protein. Following killing of the animals and necropsy bacteriological and histological examinations of different organs were performed 4, 5 or 6 h after inoculation. Results: Clearance of bacteria from the blood was completed within the first 2 h in some of the pigs and the highest bacterial load was recorded in the lungs as compared to the spleen, liver and bones. This probably was a consequence of both the intravenous route of inoculation and the presence of pulmonary intravascular macrophages. Inoculation of bacteria induced formation of acute microabscesses in the lungs, spleen and liver, but not in the kidneys or bones. No generalized inflammatory response was recorded, i.e. IL-6 was not detected in the blood and C-reactive protein did not increase, probably because of the short time course of the study. Conclusion: This study demonstrates the successful induction of acute pyemia (microabscesses), and forms a basis for future experiments that should include inoculation with strains of S. aureus isolated from man and an extension of the timeframe aiming at inducing sepsis, severe sepsis and septic shock.

U2 - 10.1186/1751-0147-51-14

DO - 10.1186/1751-0147-51-14

JO - Acta Veterinaria Scandinavica (Online Edition)

JF - Acta Veterinaria Scandinavica (Online Edition)

SN - 1751-0147

VL - 51

SP - 14

ER -